STEMdiff™ Definitive Endoderm Kit (TeSR™-E8™ Optimized)
Defined animal component-free medium for the differentiation of human ES and iPS cells maintained in TeSR™-E8™ to definitive endoderm
STEMdiff™ Definitive Endoderm Kit (TeSR™-E8™ Optimized)
Defined animal component-free medium for the differentiation of human ES and iPS cells maintained in TeSR™-E8™ to definitive endoderm
Product Advantages
- Defined, serum- and animal component-free medium for the differentiation of human ES and iPS cells to definitive endoderm in a complete, ready-to-use format
- Efficient and reproducible differentiation of multiple ES cell and iPS cell lines
- Generates definitive endoderm cells capable of further differentiation to pancreatic, hepatic, intestinal and pulmonary cell lineages
- Optimized for use with cells maintained in TeSR™-E8™ cell culture medium
What's Included
- STEMdiff™ Endoderm Basal Medium, 100 mL
- STEMdiff™ Definitive Endoderm Supplement MR (100X), 0.35 mL
- STEMdiff™ Definitive Endoderm Supplement CJ (100X), 1.1 mL
- STEMdiff™ Definitive Endoderm TeSR™-E8™ Supplement, 7 mL
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Gentle Cell Dissociation ReagentcGMP, enzyme-free cell dissociation reagent
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Vitronectin XF™Defined, xeno-free matrix that supports the growth and differentiation of human pluripotent stem cells under serum-free, feeder-free conditions
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Y-27632 (Dihydrochloride)RHO/ROCK pathway inhibitor; Inhibits ROCK1 and ROCK2
Overview
Optimized for differentiation of cells maintained in TeSR™-E8™ . For differentiation of cells maintained in mTeSR™1, please see the STEMdiff™ Definitive Endoderm Kit.
Data Figures
Figure 1. Definitive Endoderm Differentiation is Efficient Across Multiple ES and iPS Cell Lines Maintained in Either mTeSR™1 or TeSR™-E8™ Maintenance Media
Quantitative analysis of definitive endoderm formation in multiple hES (H1 and H9) and hiPS (WLS-4D1 and STiPS-M001) lines as measured by co-expression of CXCR4 and SOX17. Cells maintained in mTeSR™1 or TeSR™-E8™ were differentiated using the STEMdiff™ Definitive Endoderm Kit or the STEMdiff™ Definitive Endoderm Kit (TeSR™-E8™-Optimized), respectively. Data are expressed as the mean percentage of cells expressing both markers. Error bars indicate SEM.
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Resources and Publications
Educational Materials (7)
Related Products
Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
