STEMdiff™ Definitive Endoderm Kit (TeSR™-E8™ Optimized)

Defined animal component-free medium for the differentiation of human ES and iPS cells maintained in TeSR™-E8™ to definitive endoderm
STEMdiff™ Definitive Endoderm Kit (TeSR™-E8™ Optimized)

Defined animal component-free medium for the differentiation of human ES and iPS cells maintained in TeSR™-E8™ to definitive endoderm

1 Kit
Catalog # 05115
478 EUR
Required Products
  1. Gentle Cell Dissociation Reagent
    Gentle Cell Dissociation Reagent

    cGMP, enzyme-free cell dissociation reagent

  2. Vitronectin XF™
    Vitronectin XF™

    Defined, xeno-free matrix that supports the growth and differentiation of human pluripotent stem cells under serum-free, feeder-free conditions

  3. Y-27632 (Dihydrochloride)
    Y-27632

    RHO/ROCK pathway inhibitor; Inhibits ROCK1 and ROCK2

Overview

STEMdiff™ Definitive Endoderm Kit (TeSR™-E8™ Optimized) is a complete, serum- and animal component-free medium and supplement kit that supports highly efficient differentiation of human ES cells and iPS cells that have been maintained in TeSR™-E8™ to definitive endoderm. Cells differentiated using the STEMdiff™ Definitive Endoderm Kit (TeSR™-E8™ Optimized) express high levels of endoderm markers, including CD184 (CXCR4) and SOX17. When used in combination with Vitronectin XF™, the STEMdiff™ Definitive Endoderm Kit (TeSR™-E8™ Optimized) provides a completely xeno-free workflow for the generation of multipotent definitive endoderm.

Optimized for differentiation of cells maintained in TeSR™-E8™ . For differentiation of cells maintained in mTeSR™1, please see the STEMdiff™ Definitive Endoderm Kit.
Advantages
• Defined, serum- and animal component-free medium for the differentiation of human ES and iPS cells to definitive endoderm in a complete, ready-to-use format
• Efficient and reproducible differentiation of multiple ES cell and iPS cell lines
• Generates definitive endoderm cells capable of further differentiation to pancreatic, hepatic, intestinal and pulmonary cell lineages
• Optimized for use with cells maintained in TeSR™-E8™ cell culture medium
Components
  • STEMdiff™ Endoderm Basal Medium, 100 mL
  • STEMdiff™ Definitive Endoderm Supplement MR (100X), 0.35 mL
  • STEMdiff™ Definitive Endoderm Supplement CJ (100X), 1.1 mL
  • STEMdiff™ Definitive Endoderm TeSR™-E8™ Supplement, 7 mL
Subtype
Specialized Media
Cell Type
Airway Cells, Endoderm, PSC-Derived, Hepatic Cells, Intestinal Cells, Pancreatic Cells, Pluripotent Stem Cells
Species
Human
Application
Cell Culture, Differentiation
Brand
STEMdiff
Area of Interest
Cancer, Epithelial Cell Biology, Stem Cell Biology
Formulation
Animal Component-Free, Serum-Free

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
STEMdiff™ Definitive Endoderm Kit (TeSR™-E8™ Optimized)
Catalog #
05115
Lot #
Component #05113: Lot 18D89658 and higher
Language
English
Document Type
Safety Data Sheet 1
Product Name
STEMdiff™ Definitive Endoderm Kit (TeSR™-E8™ Optimized)
Catalog #
05115
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
STEMdiff™ Definitive Endoderm Kit (TeSR™-E8™ Optimized)
Catalog #
05115
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
STEMdiff™ Definitive Endoderm Kit (TeSR™-E8™ Optimized)
Catalog #
05115
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
STEMdiff™ Definitive Endoderm Kit (TeSR™-E8™ Optimized)
Catalog #
05115
Lot #
All
Language
English
Document Type
Safety Data Sheet 5
Product Name
STEMdiff™ Definitive Endoderm Kit (TeSR™-E8™ Optimized)
Catalog #
05115
Lot #
All
Language
English

Educational Materials (7)

Brochure
STEMdiff™ Definitive Endoderm Kit
Brochure
Products for Human Pluripotent Stem Cells
Brochure
qPCR Arrays for Cell Characterization
Wallchart
Directed Differentiation of Pluripotent Stem Cells
Webinar
Derivation of Metabolically Active Hepatocytes from Human Pluripotent Stem Cells
36:59
Derivation of Metabolically Active Hepatocytes from Human Pluripotent Stem Cells
Scientific Poster
Highly Efficient Differentiation of Human Pluripotent Stem Cells to Multipotent Definitive Endoderm Using STEMdiff™ Definitive Endoderm Culture System
Scientific Poster
Highly Efficient Differentiation of Human Pluripotent Stem Cells Maintained in TeSR™-E8™ to Definitive Endoderm

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Definitive Endoderm Differentiation is Efficient Across Multiple ES and iPS Cell Lines Maintained in Either mTeSR™1 or TeSR™-E8™ Maintenance Media

Figure 1. Definitive Endoderm Differentiation is Efficient Across Multiple ES and iPS Cell Lines Maintained in Either mTeSR™1 or TeSR™-E8™ Maintenance Media

Quantitative analysis of definitive endoderm formation in multiple hES (H1 and H9) and hiPS (WLS-4D1 and STiPS-M001) lines as measured by co-expression of CXCR4 and SOX17. Cells maintained in mTeSR™1 or TeSR™-E8™ were differentiated using the STEMdiff™ Definitive Endoderm Kit or the STEMdiff™ Definitive Endoderm Kit (TeSR™-E8™-Optimized), respectively. Data are expressed as the mean percentage of cells expressing both markers. Error bars indicate SEM.

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