IntestiCult™ Organoid Growth Medium (Human)

Cell culture medium for establishment and maintenance of human intestinal organoids

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IntestiCult™ Organoid Growth Medium (Human)

Cell culture medium for establishment and maintenance of human intestinal organoids

1 Kit
Catalog #06010
449 USD

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Overview

IntestiCult™ Organoid Growth Medium (Human) is a complete cell culture medium for efficient establishment and long-term maintenance of intestinal organoids derived from human intestinal crypts. Intestinal organoids provide a convenient in vitro organotypic culture system for studying the intestinal epithelium. The organoids incorporate a functional lumen enclosed by a polarized epithelial cell layer containing known cell types of the adult intestinal epithelium. Isolated intestinal crypts rapidly form organoids when cultured in IntestiCult™ Organoid Growth Medium (Human). Maturing intestinal organoids establish protruding buds, resembling crypts of the in vivo intestine. Applications of intestinal organoid cultures include studying the development and function of intestinal epithelium, modeling intestinal diseases, and performing targeted small molecule screens. Intestinal organoid cultures can also be used for investigation of adult stem cell properties and for regenerative therapy approaches.
This product is a prototype manufactured under ISO 13485, Medical Devices. Stability has not yet been established.
Advantages:
• Convenient, in vitro system that recapitulates the identity and organization of the adult intestinal epithelium, including intra- and intercellular signaling, self-propagating stem cell niche, and functional transport into and out of the lumen
• Complete medium formulation that delivers consistent results
• Enables generation of intestinal organoids in one week
• Easy-to-use format and optimized protocol
Components:
  • IntestiCult OGM Human Component A, 50 mL
  • IntestiCult OGM Human Component B, 50 mL
Subtype:
Specialized Media
Cell Type:
Intestinal Cells
Species:
Human
Application:
Cell Culture; Differentiation; Expansion; Maintenance; Organoid Culture
Brand:
IntestiCult
Area of Interest:
Disease Modeling; Drug Discovery and Toxicity Testing; Epithelial Cell Biology; Stem Cell Biology

Scientific Resources

Educational Materials

(6)

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area Workflow Stages for
Workflow Stages

Data and Publications

Data

Figure 1. Primary Organoids Grown in IntestiCult™ Organoid Growth Medium (Human) are Fully Mature After 10-14 Days in Culture

Primary organoids were cultured from human colonic biopsy samples and grown in IntestiCult Organoid Growth Medium (Human). Organoids were imaged after (A) two days, (B) six days, (C) eight days and (D) ten days growth.

Figure 2. Organoids Grown in IntestiCult™ Organoid Growth Medium (Human) Display Markers of Human Intestinal Epithelial Cells

Immunofluorescence of organoids grown in IntestiCult™ Organoid Growth Medium (Human) showing colocalization of (A) DAPI, (B) EPCAM and (C) Ki67. (D) A merged image shows the position of actively proliferating (Ki67+) intestinal stem cells within the epithelial layer (EPCAM+).

Figure 3. Forskolin-Induced Swelling of Organoids Grown in IntestiCult™ Organoid Growth Medium (Human)

Organoids were treated with (A) 5 μM Forskolin or (B) DMSO and organoid area was measured at 0 minutes and 120 minutes. (C)Forskolin-treated organoids increased in size 33.5 ± 3.8% compared to 7.5 ± 0.8% for DMSO-treated organoids.

STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.
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