Heparin Solution

Cell culture supplement

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Heparin Solution

Cell culture supplement

2 mL
Catalog #07980
37 USD

Overview

Heparin is a mucopolysaccharide with anticoagulant properties. It supports the binding of fibroblast growth factor (FGF) to its receptor and increases the stability of FGF. Heparin is used together with epidermal growth factor (EGF) and FGF in NeuroCult™ media for the culture of embryonic rat or adult human neural stem and progenitor cells, and adult mouse neural stem and progenitor cells. For complete instructions, refer to the Technical Manual: In Vitro Proliferation and Differentiation of Neural Stem and Progenitor Cells Using NeuroCult™ (Human; Document #28724 or Mouse/Rat; Document #28725), available at www.stemcell.com or contact us to request a copy.

Heparin Solution is also required as a supplement in various other STEMCELL culture media, including MammoCult™ Human Medium Kit (Catalog #05620), EpiCult™-B Mouse Medium Kit (Catalog #05610), and EC-Cult™-XF Culture Kit (Catalog #08000).
Contains:
0.2% (2 mg/mL; 1000 IU/mL) Heparin sodium salt in phosphate-buffered saline (PBS)
Subtype:
Supplements
Cell Type:
Neural Stem and Progenitor Cells; Mammary Cells; Prostate Cells; Other
Species:
Human; Rat
Application:
Cell Culture

Scientific Resources

Product Documentation

Document Type
Product Name
Catalog #
Lot #
Language

Educational Materials

(1)

Data and Publications

Publications

(1)
Neuro-oncology 2011 SEP

Antiparasitic mebendazole shows survival benefit in 2 preclinical models of glioblastoma multiforme.

Bai R-Y et al.

Abstract

Glioblastoma multiforme (GBM) is the most common and aggressive brain cancer, and despite treatment advances, patient prognosis remains poor. During routine animal studies, we serendipitously observed that fenbendazole, a benzimidazole antihelminthic used to treat pinworm infection, inhibited brain tumor engraftment. Subsequent in vitro and in vivo experiments with benzimidazoles identified mebendazole as the more promising drug for GBM therapy. In GBM cell lines, mebendazole displayed cytotoxicity, with half-maximal inhibitory concentrations ranging from 0.1 to 0.3 µM. Mebendazole disrupted microtubule formation in GBM cells, and in vitro activity was correlated with reduced tubulin polymerization. Subsequently, we showed that mebendazole significantly extended mean survival up to 63% in syngeneic and xenograft orthotopic mouse glioma models. Mebendazole has been approved by the US Food and Drug Administration for parasitic infections, has a long track-record of safe human use, and was effective in our animal models with doses documented as safe in humans. Our findings indicate that mebendazole is a possible novel anti-brain tumor therapeutic that could be further tested in clinical trials.
STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.