hPSC Genetic Analysis Kit
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hPSC Genetic Analysis Kit -
Label for hPSC Genetic Analysis Kit
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Required Products
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Axygen® Sealing Film for Quantitative Real-Time PCR (qPCR)
Non-sterile, ultra-clear, pressure-sensitive sealing film for quantitative real-time PCR (qPCR) -
For purification of genomic DNA from cells or tissue
Overview
Specifically, the kit includes qPCR Master Mix, ROX Reference Dye, Buffer and primer/probe mixes designed to detect the critical minimal regions of these 8 commonly mutated regions along with a primer/probe mix to a control location in the genome. Additionally, a genomic DNA control is provided with demonstrated normal copy number over the regions of interest.
Our online Genetic Analysis Application ( www.stemcell.com/geneticanalysisapp ) is designed to help with data analysis and interpretation: simply input qPCR data and the tool will perform statistical analyses, assist with data interpretation, and provide visual representation of the data.
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Human Pluripotent Stem Cell Trilineage Differentiation qPCR Array
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Scientific Resources
Product Documentation
Educational Materials
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Product Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Data and Publications
Data
Figure 1. The hPSC Genetic Analysis Kit Identifies Chromosome 12 Trisomy
Chromosome 12 trisomy in WLS-1C human iPS cell line is (A) detected using the hPSC Genetic Analysis Kit (orange bar) and (B) confirmed by G-banding.
Figure 2. The hPSC Genetic Analysis Kit Identifies Chromosome 1 Duplication via Unbalanced Translocation
Unbalanced rearrangement of chromosome 1 in the WLS-1C human iPS cell line in which an extra copy of the long (q) arm of chromosome 1 translocated to the short arm (p) of chromosome 21 was (A) detected using the hPSC Genetic Analysis Kit (orange bar) and (B) confirmed by G-banding.
Figure 3. The hPSC Genetic Analysis Kit Identifies Chromosome 20q11.21 Duplication
Chromosome 20q duplication in WLS-1C human iPS cell line is (A) detected using the hPSC Genetic Analysis Kit (orange bar), (B) undetected by G-banding, and (C) confirmed by fluorescent in situ hybridization using probes for 20p11 (green) and 20q11.21 (red).
Figure 4. The hPSC Genetic Analysis Kit Identifies Abnormalities in Cultures with Approximately 30% Mosaicism
Genetically normal WLS-1C human iPS cells were mixed in the indicated ratios with WLS-1C human iPS cells containing a chromosome 20q duplication. Cultures with approximately 30% genetically abnormal cells exhibit a significantly enriched population of 20q11.21 duplication (orange bars).