hPSC Genetic Analysis Kit

qPCR analysis kit for detecting the majority of karyotypic abnormalities reported in human ES and iPS cells

New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more

hPSC Genetic Analysis Kit

qPCR analysis kit for detecting the majority of karyotypic abnormalities reported in human ES and iPS cells

hPSC Genetic Analysis Kit
1 Kit
680 USD
Catalog # 07550

qPCR analysis kit for detecting the majority of karyotypic abnormalities reported in human ES and iPS cells

What's Included

• qPCR Master Mix (2X), 3 mL
• ROX Reference Dye, 0.2 mL
• Chr 1q Genetic Assay, 60 Rxn
• Chr 4p Genetic Assay, 60 Rxn
• Chr 8q Genetic Assay, 60 Rxn
• Chr 10p Genetic Assay, 60 Rxn
• Chr 12p Genetic Assay, 60 Rxn
• Chr 17q Genetic Assay, 60 Rxn
• Chr 18q Genetic Assay, 60 Rxn
• Chr 20q Genetic Assay, 60 Rxn
• Chr Xp Genetic Assay, 60 Rxn
• Genomic DNA Control, 15 µL
• TE Buffer, 1 mL
New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more

Overview

Quickly and cost-effectively detect the 8 most common karyotypic abnormalities reported in human pluripotent stem cells (hPSCs).

This qPCR-based kit enables the genetic screening of multiple hPSC lines and contains enough material to analyze 20 individual samples in triplicate. It uses double-quenched probes with a 5-carboxyfluorescein (5-FAM) dye to give superior performance over other, single-quenched probes. The kit includes qPCR Master Mix, ROX Reference Dye, Buffer, and primer/probe mixes designed to detect the critical minimal regions of the 8 most commonly mutated regions, as well as a primer/probe mix to a control location in the genome. A genomic DNA control is also provided with demonstrated normal copy number over the regions of interest.

To understand your results, input qPCR data into our online Genetic Analysis App, which will perform statistical analyses, assist with data interpretation, and provide visual representation of the data.
Cell Type
Pluripotent Stem Cells
Species
Human
Area of Interest
Disease Modeling, Drug Discovery and Toxicity Testing, Stem Cell Biology

Data Figures

Figure 1. The hPSC Genetic Analysis Kit Identifies Chromosome 12 Trisomy

Chromosome 12 trisomy in WLS-1C human iPS cell line is (A) detected using the hPSC Genetic Analysis Kit (orange bar) and (B) confirmed by G-banding.

Figure 2. The hPSC Genetic Analysis Kit Identifies Chromosome 1 Duplication via Unbalanced Translocation

Unbalanced rearrangement of chromosome 1 in the WLS-1C human iPS cell line in which an extra copy of the long (q) arm of chromosome 1 translocated to the short arm (p) of chromosome 21 was (A) detected using the hPSC Genetic Analysis Kit (orange bar) and (B) confirmed by G-banding.

Figure 3. The hPSC Genetic Analysis Kit Identifies Chromosome 20q11.21 Duplication

Chromosome 20q duplication in WLS-1C human iPS cell line is (A) detected using the hPSC Genetic Analysis Kit (orange bar), (B) undetected by G-banding, and (C) confirmed by fluorescent in situ hybridization using probes for 20p11 (green) and 20q11.21 (red).

Figure 4. The hPSC Genetic Analysis Kit Identifies Abnormalities in Cultures with Approximately 30% Mosaicism

Genetically normal WLS-1C human iPS cells were mixed in the indicated ratios with WLS-1C human iPS cells containing a chromosome 20q duplication. Cultures with approximately 30% genetically abnormal cells exhibit a significantly enriched population of 20q11.21 duplication (orange bars).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

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Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Resources and Publications

Educational Materials (34)

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