hPSC Genetic Analysis Kit
qPCR analysis kit for detecting the majority of karyotypic abnormalities reported in human ES and iPS cells
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Genomic DNA Purification KitFor purification of genomic DNA from cells or tissue
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Genomic DNA Purification KitFor purification of genomic DNA from cells or tissue
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Axygen® Sealing Film for Quantitative Real-T...Non-sterile, ultra-clear, pressure-sensitive sealing film for quantitative real-time PCR (qPCR)
Overview
This qPCR-based kit enables the genetic screening of multiple hPSC lines and contains enough material to analyze 20 individual samples in triplicate. It uses double-quenched probes with a 5-carboxyfluorescein (5-FAM) dye to give superior performance over other, single-quenched probes. The kit includes qPCR Master Mix, ROX Reference Dye, Buffer, and primer/probe mixes designed to detect the critical minimal regions of the 8 most commonly mutated regions, as well as a primer/probe mix to a control location in the genome. A genomic DNA control is also provided with demonstrated normal copy number over the regions of interest.
To understand your results, input qPCR data into our online Genetic Analysis App, which will perform statistical analyses, assist with data interpretation, and provide visual representation of the data.
Data Figures
Figure 1. The hPSC Genetic Analysis Kit Identifies Chromosome 12 Trisomy
Chromosome 12 trisomy in WLS-1C human iPS cell line is (A) detected using the hPSC Genetic Analysis Kit (orange bar) and (B) confirmed by G-banding.
Figure 2. The hPSC Genetic Analysis Kit Identifies Chromosome 1 Duplication via Unbalanced Translocation
Unbalanced rearrangement of chromosome 1 in the WLS-1C human iPS cell line in which an extra copy of the long (q) arm of chromosome 1 translocated to the short arm (p) of chromosome 21 was (A) detected using the hPSC Genetic Analysis Kit (orange bar) and (B) confirmed by G-banding.
Figure 3. The hPSC Genetic Analysis Kit Identifies Chromosome 20q11.21 Duplication
Chromosome 20q duplication in WLS-1C human iPS cell line is (A) detected using the hPSC Genetic Analysis Kit (orange bar), (B) undetected by G-banding, and (C) confirmed by fluorescent in situ hybridization using probes for 20p11 (green) and 20q11.21 (red).
Figure 4. The hPSC Genetic Analysis Kit Identifies Abnormalities in Cultures with Approximately 30% Mosaicism
Genetically normal WLS-1C human iPS cells were mixed in the indicated ratios with WLS-1C human iPS cells containing a chromosome 20q duplication. Cultures with approximately 30% genetically abnormal cells exhibit a significantly enriched population of 20q11.21 duplication (orange bars).
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Resources and Publications
Educational Materials (34)
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Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
