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Propidium Iodide

Cell viability dye (DNA-labeling dye)

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Propidium Iodide

Cell viability dye (DNA-labeling dye)

From: 94 USD
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Cell viability dye (DNA-labeling dye)
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Overview

Propidium Iodide (PI) is a red-fluorescent cell viability dye which is excluded from live cells with intact membranes, but penetrates dead or damaged cells and binds to DNA and RNA by intercalating between the bases. It is widely used as a counterstain to differentiate and exclude non-viable cells in flow cytometric analyses, and can be excited using blue (488 nm), green (532 nm), or yellow-green (561 nm) laser lines, with detection in the FL2 or FL3 channels. PI is used in DNA fluorescence imaging applications to discriminate early and late stages of apoptosis, to study cell‐mediated cytotoxicity, and for chromosome analysis. It is also commonly used in quantitative DNA assays.
Alternative Names
3,8-Diamino-5-{3-[diethyl(methyl)ammonio]propyl}-6-phenylphenanthridinium diiodide; PI; Propidium diiodide
Cell Type
Other
Species
Human, Mouse, Non-Human Primate, Other, Rat
Application
Flow Cytometry
Area of Interest
Immunology, Neuroscience, Stem Cell Biology
CAS Number
25535-16-4
Chemical Formula
C₂₇H₃₄N₄ · 2I
Molecular Weight
668.4 g/mol

Data Figures

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Propidium Iodide

Figure 1. (A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Propidium Iodide

(B) Chemical structure of Propidium Iodide.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Propidium Iodide
Catalog #
75002
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Propidium Iodide
Catalog #
75002
Lot #
All
Language
English

Applications

Resources and Publications

Educational Materials (1)

Human Immune Cytokines
Wallchart
Human Immune Cytokines

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Quality Statement:

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.

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