Propidium Iodide
Cell viability dye (DNA-labeling dye)
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Propidium Iodide -
Label for Propidium Iodide
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Dulbecco’s phosphate-buffered saline without calcium and magnesium
Overview
Propidium Iodide (PI) is a red-fluorescent cell viability dye which is excluded from live cells with intact membranes, but penetrates dead or damaged cells and binds to DNA and RNA by intercalating between the bases. It is widely used as a counterstain to differentiate and exclude non-viable cells in flow cytometric analyses, and can be excited using blue (488 nm), green (532 nm), or yellow-green (561 nm) laser lines, with detection in the FL2 or FL3 channels. PI is used in DNA fluorescence imaging applications to discriminate early and late stages of apoptosis, to study cell‐mediated cytotoxicity, and for chromosome analysis. It is also commonly used in quantitative DNA assays.
Application:
Flow Cytometry
Area of Interest:
Neuroscience; Immunology; Stem Cell Biology
Chemical Formula:
C₂₇H₃₄N₄ · 2I
Molecular Weight:
668.4 g/mol
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Cell viability dye (DNA-labeling dye) -
For the identification, evaluation and isolation of stem and progenitor cells expressing high levels of ALDH
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This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
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Data
Figure 1. (A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Propidium Iodide
(B) Chemical structure of Propidium Iodide.
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.