STEMdiff™ Sensory Neuron Differentiation Kit

Differentiation kit for the generation of sensory neuronal precursors from human iPS cell-derived neural crest cells
Catalog #
100-0341_C
Differentiation kit for the generation of sensory neuronal precursors from human iPS cell-derived neural crest cells
From: 299 USD
Required Products

Overview

Generate functional, human-specific sensory neurons in a dish with this easy-to-use culture system. After obtaining pluripotent stem cell (PSC)-derived neural crest cells (NCCs) with STEMdiff™ Neural Crest Differentiation Kit, use the serum-free STEMdiff™ Sensory Neuron Differentiation Kit to generate sensory neuron precursors. STEMdiff™ Sensory Neuron Maturation Kit can then be used to maintain and mature the sensory neuron precursors. The resulting cells are BRN3A-positive and more than 70% TUJ1-positive. With the physiological glucose and osmolarity conditions provided by BrainPhys™ as the basal medium, the neurons exhibit activity in response to sensory ligands and changes in temperature. The generated population of functional human sensory neurons can be used for drug discovery and pain research applications.
Advantages
⦁ Reduce animal usage by eliminating costly dorsal root ganglia explant workflows
⦁ Differentiate sensory neurons from multiple human induced pluripotent stem (iPS) cell lines using an NCC intermediate and the STEMdiff™ Neural Crest Differentiation Kit
⦁ Streamline PSC-derived sensory neuron generation with a simple, easy-to-use media format
⦁ Obtain physiologically relevant results with integrated BrainPhys™ media supporting neuronal activity and maturation
Components
  • STEMdiff™ Sensory Neuron Differentiation Basal Medium, 100 mL
  • STEMdiff™ Sensory Neuron Differentiation Supplement, 1 mL
Subtype
Specialized Media
Cell Type
Neural Cells, PSC-Derived, Neurons
Species
Human
Application
Cell Culture, Differentiation
Brand
BrainPhys, STEMdiff
Area of Interest
Disease Modeling, Drug Discovery and Toxicity Testing, Neuroscience
Formulation
Serum-Free

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
STEMdiff™ Sensory Neuron Differentiation Kit
Catalog #
100-0341
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
STEMdiff™ Sensory Neuron Differentiation Kit
Catalog #
100-0341
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
STEMdiff™ Sensory Neuron Differentiation Kit
Catalog #
100-0341
Lot #
All
Language
English

Educational Materials (9)

Brochure
hPSC-Derived Neural Cell Research
Brochure
Products for Human Pluripotent Stem Cells
Brochure
STEMCELL Neural Product Portfolio
Wallchart
Directed Differentiation of Pluripotent Stem Cells
Wallchart
Neural Stem Cells
Webinar
The Road to Functional Human Neuronal Circuits in Vitro
56:27
The Road to Functional Human Neuronal Circuits in Vitro
Webinar
hPSC-Derived Neural Crest: Induction and Axial Patterning
54:21
hPSC-Derived Neural Crest: Induction and Axial Patterning
Scientific Poster
Efficient Generation of Human Pluripotent Stem Cells to Neural Crest Cells
Scientific Poster
Efficient Differentiation of Human Pluripotent Stem Cells to Sensory Neurons

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Experimental Protocol for STEMdiff™ Sensory Neuron Differentiation and Maturation Kits

Figure 1. Schematic for the STEMdiff™ Sensory Neuron Culture System Protocol

Sensory neuron precursors can be generated in 6 days from hPSC-derived neural crest cells. For the generation of neural crest cells, see documentation for STEMdiff™ Neural Crest Differentiation Kit (Catalog #08610). For the maturation of sensory neuron precursors to sensory neurons, see the PIS.

Figure 2. STEMdiff™ Sensory Neuron Kits Promote Differentiation Across Multiple Embryonic Stem and Induced Pluripotent Stem Cell Lines

NCCs generated from hPSCs in mTeSR™ Plus using the STEMdiff™ Neural Crest Differentiation Kit were differentiated and matured to sensory neurons using the STEMdiff™ Sensory Neuron Differentiation and Maturation Kits. (A) Sensory neurons were generated after hPSC-derived NCCs were cultured with the STEMdiff™ Sensory Neuron Differentiation Kit for 6 days and then the STEMdiff™ Sensory Neuron Maturation Kit for 6 days. The resulting cultures contain a population of cells expressing sensory neuron markers peripherin (green) and BRN3A (red) along with (B) neuronal marker class III β-tubulin (TUJ1, red). (C) Midbrain neuron controls generated with STEMdiff™ Midbrain Neuron Differentiation and Maturation Kits do not have detectable peripherin (green) or BRN3A (red) expression, although they express (D) neuronal marker class III β-tubulin (TUJ1, red). Nuclei are labeled with DAPI (blue). Human ES and iPS cell lines were maintained in either mTeSR™1, TeSR™-E8™, or mTeSR™ Plus and differentiated with STEMdiff™ Neural Crest Differentiation Kit, followed by STEMdiff™ Sensory Neuron Differentiation and Maturation Kits. The percentage expression of (E) BRN3A+ and (F) TUJ1+ cells in the resulting cultures was quantified. This differentiation generated BRN3A+ sensory neurons (25.3% ± 6.9%, mean ± SEM; n=7 cell lines, 3 - 23 replicates per condition) that expressed neuronal marker class III β-tubulin (TUJ1; 90.3% ± 4.1%, mean ± SEM; n=4 cell lines, 3 - 12 replicates per condition). Numbers are % positive over total DAPI in a tiled image. NCCs = neural crest cells; hPSCs = human pluripotent stem cells; ES = embryonic stem; iPS = induced pluripotent stem

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