Pluripotent Stem Cell Training
Training to support the culture of hPSCs and their differentiation towards cerebral organoids, intestinal organoids, cardiomyocytes, or hematopoietic progenitors
Note on COVID-19 restrictions: STEMCELL Technologies is following local governmental advice and will not conduct in-person training events until it is safe to do so. We are continually monitoring the situation and hope to resume our hands-on offerings soon. In the meantime, we continue to support your training needs with live virtual and on-demand training courses. For our current course availability, please see our training schedule.
Request Pricing
Thank you for your interest in this product. Please provide us with your contact information and your local representative will contact you with a customized quote. Where appropriate, they can also assist you with a(n):
Estimated delivery time for your area
Product sample or exclusive offer
In-lab demonstration
By submitting this form, you are providing your consent to STEMCELL Technologies Canada Inc. and its subsidiaries and affiliates (“STEMCELL”) to collect and use your information, and send you newsletters and emails in accordance with our privacy policy. Please contact us with any questions that you may have. You can unsubscribe or change your email preferences at any time.
Note on COVID-19 restrictions: STEMCELL Technologies is following local governmental advice and will not conduct in-person training events until it is safe to do so. We are continually monitoring the situation and hope to resume our hands-on offerings soon. In the meantime, we continue to support your training needs with live virtual and on-demand training courses. For our current course availability, please see our training schedule.
Overview
Learn the techniques and protocols to successfully derive and maintain high-quality human induced pluripotent stem (iPS) cells from somatic cells and their differentiation towards specialized cell types.
Customize your training by choosing from the course topics available:
- Reprogramming somatic cells to iPS cells
- Maintaining iPS cells and human embryonic stem (ES) cells
- *Differentiating ES and iPS cells towards one of the following:
- Cardiomyocytes (Option A)
- Hematopoietic progenitor cells (Option B)
- Neural cell types including cerebral organoids (Option C)
- Intestinal Organoids (Option D)
*Select your differentiation topic of choice (Option A, B, C, or D) in the Sign Up form.
See Agenda for more information.
Participants Will Receive
- Electronic and hardcopy versions of instructional materials
- Tote bag with sample-sized products (where applicable)
- Certificate following successful completion of the course
- Lunch and refreshments on each day and dinner hosted on one of the evenings
Training and Education
Hands-On Training
Stem Cell Biology; Drug Discovery and Toxicity Testing
Embryonic Stem Cells and iPS Cells, Neural Cells, PSC-Derived, Hematopoietic Stem and Progenitor Cells, Intestinal Cells, Cardiomyocytes, PSC-Derived
Human
Canada:
STEMCELL Technologies
1618 Station Street
Vancouver, CA V6A 1B6
STEMCELL Technologies
1618 Station Street
Vancouver, CA V6A 1B6
United Kingdom:
STEMCELL Technologies UK Ltd.
Building 7100, Cambridge Research Park,
Beach Drive, Waterbeach
Cambridge, UK, CB25 9TL
STEMCELL Technologies UK Ltd.
Building 7100, Cambridge Research Park,
Beach Drive, Waterbeach
Cambridge, UK, CB25 9TL
The Netherlands:
Leiden University Medical Center
Building 2
Einthovenweg 20, 2333 ZC Leiden
Leiden, NL
Leiden University Medical Center
Building 2
Einthovenweg 20, 2333 ZC Leiden
Leiden, NL
Speakers
Speakers will vary depending on course topic, date, and location.
Arwen Hunter, PhD
Senior Scientist (Pluripotent Group), Research and Development
STEMCELL Technologies
STEMCELL Technologies
Leon Chew, MSc
Scientist (Neural Group), Research and Development
STEMCELL Technologies
STEMCELL Technologies
Vincenzo Macri, PhD
Senior Scientist (Myogenic Group), Research and Development
STEMCELL Technologies
STEMCELL Technologies
Wing Chang, PhD
Director, Research and Development UK
STEMCELL Technologies
STEMCELL Technologies
Phillip Kramer, PhD
Senior Scientist (Intestinal Group), Research and Development
STEMCELL Technologies
STEMCELL Technologies
Agenda
Note that each day will end at approximately 4:30 pm with the course dinner taking place on the evening of Maintaining Human Pluripotent Stem Cells (hPSCs)
All course options followed by wrap-up which includes a quiz, evaluation, and certificate presentation.
Reprogramming of Somatic Cells to Induced Pluripotent Stem Cells (iPS Cells)
-
Lecture: Isolation and Reprogramming of Somatic Cells to iPS Cells
-
Practical Session: Isolation of Peripheral Blood Mononuclear Cells (PBMCs)
- Overview of EasySep™ cell separation system
- Demonstrate preparation of PBMCs using SepMate™
- Observe culture-expanded blood cells in StemSpan™ SFEM
-
Practical Session: Reprogramming of Somatic Cells to iPS Cells
- Demonstrate transfection of human fibroblasts using ReproRNA™-OKSGM
- Observe reprogrammed cells cultured in ReproTeSR™
-
Practical Session: Selection and Picking of iPS Cell Colonies
Maintaining Human Pluripotent Stem Cells (hPSCs)
-
Lecture: Maintaining and Assessing High-Quality hPSC Cultures
-
Lecture: Genome Editing with CRISPR-Cas9
-
Practical Session: Evaluating Quality of hPSC Cultures and Plate Coating
- Observe time course of hPSC cultures and review colony morphology
- Practice plate coating with Matrigel® or Vitronectin XF™
-
Practical Session: Passaging and Long-Term Maintenance of hPSCs
- Practice enzyme-free passaging of hPSCs using Gentle Cell Dissociation Reagent or ReLeSR™
-
Practical Session: Cryopreservation and Thawing of hPSCs
-
Dinner (Courtesy of STEMCELL Technologies)
Differentiating hPSCs (Select Option in Sign-Up Form)
Guest Lecture by Christine Mummery: Microphysiological Systems Based on hiPSC to Study Cardiovascular Disease (Available in The Netherlands session only)
Option A: Differentiation towards Cardiomyocytes (Available in Cambridge, UK and Vancouver, CA only)
-
Lecture: Modeling Long QT Type 2 Syndrome using the STEMdiff™ Cardiomyocyte System
-
Practical Session: Evaluation of the Quality of hPSC Cultures after Passaging
-
Practical Session: Preparation of hPSCs as Single Cells for Cardiomyocyte Differentiation
-
Practical Session: Observation of hPSC-Derived Cardiomyocytes
- Observe time course of hPSC-derived cardiomyocytes
- Dissociation and replating of hPSC-derived cardiomyocytes
-
Practical Session: Cryopreservation and Thawing of hPSC-Derived Cardiomyocytes and Downstream Applications
Option B: Differentiation towards Hematopoietic Progenitor Cells (HPCs)
-
Lecture: Differentiation of hPSCs towards Hematopoietic Progenitor Cells
-
Practical Session: Evaluation of the Quality of hPSC Cultures after Passaging
-
Practical Session: Observation of hPSC-Derived HPCs
-
Practical Session: HPCs for Downstream Applications
- Harvest HPCs from culture supernatant
- Analyze hPSC-derived HPCs using the colony-forming unit (CFU) assay
-
Practical Session: Identification and Counting of Colonies in the CFU Assay
Option C: Differentiation towards Neural Cell Types Including Cerebral Organoids
-
Lecture: Differentiation of hPSCs Towards Neural Progenitors and Neural Cell Types
-
Practical Session: Generation of Embryoid Bodies (EBs) Using AggreWell™ and Preparation of Single Cell hPSCs for Monolayer-Based Protocols
-
Practical Session: Differentiation of hPSCs to NPCs
- Examine neural aggregates in AggreWell™ and harvest EBs
- Assess neural induction by scoring neural rosette formation
-
Lecture: Using Standardized Reagents to Generate Cerebral Organoids from hPSCs
-
Practical Session: Embed EBs in Matrigel® to Generate Cerebral Organoids
Option D: Differentiation towards Intestinal Organoids (Available in Cambridge, UK and Vancouver, CA only)
-
Lecture: Differentiation of hPSCs Towards Intestinal Organoids
-
Practical Session: Evaluate the Quality of hPSC Cultures after Passaging from Previous Day
-
Lecture: Applications of hPSC-derived Intestinal Organoids
-
Practical Session: Differentiation of hPSC Towards Intestinal Organoids
- Observe time course of hPSC cultures to evaluate differentiation efficiency
-
Practical Session: Harvest and Embed hPSC-Derived Mid-/Hind-gut Spheroids
- Embed spheroids in Matrigel®
- Observe hPSC-derived intestinal organoids at passage 0
-
Practical Session: Passage hPSC-Derived Intestinal Organoids
- Observe hPSC-derived intestinal organoids
- Passage and maintain hPSC-derived intestinal organoids
Accommodation
Vancouver, CA
Pan Pacific Vancouver
300 - 999 Canada Place, Vancouver, BC V6C 3B5
Sheraton Wall Centre
1088 Burrard St, Vancouver, BC V6Z 2R9
Executive Hotel Le Soleil
567 Hornby St, Vancouver, BC V6C 2E8
Cambridge, UK (Transport included from this location to and from training venue - Cambridge UK only)
University Arms Parker's Tavern
Regent Street Cambridge CB2 1AD
Leiden, NL
Holiday Inn Leiden
Haagse Schouwweg 10, 2332 KG, Leiden, Netherlands
IBIS Leiden Centre
Stationsplein 240-242, 2312 AR Leiden, Netherlands
City Resort Hotel Leiden
Bargelaan 180, 2333 CW Leiden, Netherlands
Please contact education@stemcell.com for more information on seasonal rates (when applicable).
Policy
Travel Visas
Citizens may require a visa in order to enter the country as visitors. Please contact us at education@stemcell.com if you require a letter of invitation for your application. Note that travel visas may take up to 30 days to process.
United Kingdom
Netherlands
Travel Visa Requirements
CanadaUnited Kingdom
Netherlands
Cancellation
A 90% refund will be issued if notification is received in writing more than 30 days in advance of the course start date. No refund will be given with less than 30 days’ notice unless we are able to find another participant to fill the spot.
STEMCELL Technologies reserves the right to cancel the course if we do not receive a minimum number of registrations 30 days in advance of the start date. Should the course be cancelled, your payment will be refunded in full. We recommend that you purchase travel insurance with your flights to cover the possibility of the course being cancelled.
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages
Workflow Stages for
Human Pluripotent Stem Cell Research
Workflow Stages for
Hematopoietic Stem and Progenitor Cell Research
Workflow Stages for
hPSC-Derived Neural Cell Research
Workflow Stages for
Intestinal Epithelial Cell Research
Workflow Stages for
Myogenic Stem and Progenitor Cell Research
Workflow Stages for
PSC Quality Control
Resources and Publications
Educational Materials (14)
Related Products
Related Products
Quality Statement:
STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.
