Human Recombinant TGF-beta 1 (Cell Culture Ready)

Ready-to-use transforming growth factor beta 1
Human Recombinant TGF-beta 1 (Cell Culture Ready)

Transforming growth factor beta 1

1 mL
Catalog # 100-0856
999 USD

Overview

Conveniently and consistently add transforming growth factor beta 1 (TGF-ꞵ1) to your cultures with this cell culture-ready formulation. This human recombinant TGF-ꞵ1 is precisely reconstituted to 0.1 mg/ml in 100 mM acetic acid and requires no additional preparation, improving reproducibility. Endotoxin levels are verified, using the LAL method to ensure consistency for use across multiple applications. A member of the TGF-β superfamily, TGF-β1 regulates diverse cellular phenotypes. TGF-β1 binds to serine-threonine kinase type I and II receptors and activates signal transduction through SMAD2/3 proteins.

TGF-β1 has been used for:
hPSC CULTURE MAINTENANCE
● Together with basic fibroblast growth factor (bFGF), supports the culture of undifferentiated human embryonic stem (ES) cells and induced pluripotent stem (iPS) cells (Amit et al. Biol Reprod, 2004).
EXOGENOUS CELL SIGNALING
● Regulate a variety of functions, including cell proliferation, differentiation, wound healing, apoptosis, and metabolism (Massagué Nat Rev Mol Cell Biol, 2000; McDowell et al. Curr Biol, 1997).
IMMUNE CELL HOMEOSTASIS
● Modulate B cell responses and mediate immunosuppressive effects on T cells and neutrophils (Letterio & Roberts Annu Rev Immunol, 1998).
Alternative Names
Cartilage-inducing factor, Differentiation inhibiting factor, TGF-beta-1, TGF-ꞵ1,Transforming growth factor beta 1
Cell Type
B Cells, Mesenchymal Stem and Progenitor Cells, Monocytes, Myeloid Cells, Neural Stem and Progenitor Cells, Pluripotent Stem Cells, T Cells, Regulatory
Area of Interest
Immunology, Neuroscience, Stem Cell Biology
Molecular Weight
12.8 kDa monomer; 25.6 kDa dimer
Purity
≥ 95% by SDS-PAGE

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
Human Recombinant TGF-beta 1 (Cell Culture Ready)
Catalog #
100-0856
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Human Recombinant TGF-beta 1 (Cell Culture Ready)
Catalog #
100-0856
Lot #
All
Language
English

Educational Materials (8)

Brochure
hPSC-Derived Neural Cell Research
Brochure
Products for Human Pluripotent Stem Cells
Brochure
Tools For Your Immunology Research
Brochure
Cytokines, Chemokines and Growth Factors
Wallchart
Directed Differentiation of Pluripotent Stem Cells
Wallchart
Small Molecules, Big Impact in Cancer Research
Wallchart
Stem Cell States: Naive to Primed Pluripotency
Wallchart
Human Immune Cytokines

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Figure 1. Human Recombinant TGF-beta 1 Is Bioactive

The biological activity of Human Recombinant TGF-beta 1 was tested by its ability to induce luciferase expression in a SMAD/TGF luciferase reporter cell line. The EC50 is defined as the effective concentration of the agonist at which luminescence is 50% of maximum. The EC50 in the example above is 0.52 ng/mL. Normalized mean % of 3 replicate wells is shown for each data point. Each assay is calibrated with a Non-WHO International Reference Material (NIBSC code: 89/514). EC50 is assay-specific; titrations should be performed to determine the optimal concentration for the intended application.

Figure 2. Human Recombinant TGF-beta 1 Has High Purity

Human Recombinant TGF-beta 1 (1 μg) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie Blue staining. Human Recombinant TGF-beta 1 is a homodimer of 12.8-kDa subunits with a predicted molecular mass of 25.6 kDa.

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