Human Recombinant TGF-beta 1

Transforming growth factor beta 1

Human Recombinant TGF-beta 1

Transforming growth factor beta 1

From: 222 USD
Catalog #
78067_C
Transforming growth factor beta 1

Overview

Add this high-quality human recombinant transforming growth factor beta 1 (TGF-β1) to your cell culture workflow. Its verified endotoxin level of ≤ 0.2 EU/μg, measured using the LAL method, allows confidence for use across multiple applications. A member of the TGF-β superfamily, TGF-β1 regulates diverse cellular phenotypes. TGF-β1 binds to serine-threonine kinase type I and II receptors and activates signal transduction through SMAD2/3 proteins.

TGF-β1 has been used for:
hPSC CULTURE MAINTENANCE
● Use TGF-β1 together with basic fibroblast growth factor (bFGF) to support the culture of undifferentiated human embryonic stem (ES) cells and induced pluripotent stem (iPS) cells (Amit et al. Biol Reprod, 2004).
EXOGENOUS CELL SIGNALING
● Regulate a variety of functions, including cell proliferation, differentiation, and metabolism (Massagué Nat Rev Mol Cell Biol, 2000; McDowell et al. Curr Biol, 1997).
IMMUNE CELL HOMEOSTASIS
● Modulate B cell responses and mediate immunosuppressive effects on T cells and neutrophils (Letterio & Roberts Annu Rev Immunol, 1998).
Subtype
Cytokines, Growth Factors
Alternative Names
Cartilage-inducing factor, Differentiation inhibiting factor, Latency-associated peptide, TGFB1, TGF-beta-1, LAP, Transforming growth factor β 1
Cell Type
B Cells, Mesenchymal Stem and Progenitor Cells, Monocytes, Myeloid Cells, Neural Stem and Progenitor Cells, Pluripotent Stem Cells, T Cells, Regulatory
Species
Human
Area of Interest
Immunology, Neuroscience, Stem Cell Biology
Molecular Weight
12.8 kDa monomer, 25.6 kDa dimer
Purity
≥ 95%

Data Figures

Figure 1. Human Recombinant TGF-beta 1 Is Bioactive

The biological activity of Human Recombinant TGF-beta 1 was tested by the ability to inhibit IL-4-induced HT-2 cell proliferation. Cell proliferation was measured after 2 days of culture using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the above example is 0.17 ng/mL.

Figure 2. Human Recombinant TGF-beta 1 Has High Purity

1 μg of Human Recombinant TGF-beta 1 was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
78067, 78067.1, 78067.2, 78067.3
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
78067, 78067.1, 78067.2, 78067.3
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

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