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Data and Publications
(A) The biological activity of Human Recombinant FGF-8A was tested by its ability to promote the proliferation of 3T3 cells in the presence 1 μg/ml of heparin. Cell proliferation was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the example above is less than 500 ng/mL. (B) 2 μg of Human Recombinant FGF-8A was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining.