Propidium Iodide

Cell viability dye (DNA-labeling dye)
Propidium Iodide

Cell viability dye (DNA-labeling dye)

10 mg
Catalog # 75002
70 USD
Required Products
  1. D-PBS Without Ca++ and Mg++
    D-PBS (Without Ca++ and Mg++)

    Dulbecco’s phosphate-buffered saline without calcium and magnesium

Overview

Propidium Iodide (PI) is a red-fluorescent cell viability dye which is excluded from live cells with intact membranes, but penetrates dead or damaged cells and binds to DNA and RNA by intercalating between the bases. It is widely used as a counterstain to differentiate and exclude non-viable cells in flow cytometric analyses, and can be excited using blue (488 nm), green (532 nm), or yellow-green (561 nm) laser lines, with detection in the FL2 or FL3 channels. PI is used in DNA fluorescence imaging applications to discriminate early and late stages of apoptosis, to study cell‐mediated cytotoxicity, and for chromosome analysis. It is also commonly used in quantitative DNA assays.
Cell Type
Other
Species
Human, Mouse, Rat, Non-Human Primate, Other
Application
Flow Cytometry
Area of Interest
Immunology, Neuroscience, Stem Cell Biology
CAS Number
25535-16-4
Chemical Formula
C₂₇H₃₄N₄ · 2I
Molecular Weight
668.4 g/mol

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
Propidium Iodide
Catalog #
75002
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Propidium Iodide
Catalog #
75002
Lot #
All
Language
English

Educational Materials(1)

Wallchart
Human Immune Cytokines

Data and Publications

Data

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Propidium Iodide

Figure 1. (A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Propidium Iodide

(B) Chemical structure of Propidium Iodide.

Contact STEMCELL Technologies

Our Customer Service, Sales, and Product and Scientific Support departments in North America are available between 6 am and 5 pm Pacific Time (9 am and 8 pm Eastern Time). One of our representatives will be happy to help you by telephone or email. Please complete the form to contact us by email. A representative will get back to you shortly.
  •  

StemCell Technologies Inc. and affiliates ("STEMCELL Technologies") does not share your email address with third parties. StemCell Technologies Inc. will use your email address to confirm your identity and send you newsletters, transaction-related emails, promotional and customer service emails in accordance with our privacy policy. You can change your email preferences at any time.