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STEMdiff™ Astrocyte Differentiation Kit

Differentiation kit for generation of astrocytic precursors from human ES and iPS cell-derived neural progenitor cells

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STEMdiff™ Astrocyte Differentiation Kit

Differentiation kit for generation of astrocytic precursors from human ES and iPS cell-derived neural progenitor cells

1 Kit
Catalog #08540
176 USD

Required Products

Overview

The STEMdiff™ Astrocyte Differentiation Kit is used to rapidly and efficiently generate astrocytic precursors from neural progenitor cells derived from human pluripotent stem cells using the STEMdiff™ Neural Induction Medium (Catalog #05835) embryoid body protocol. The astrocytic precursors generated can be matured using the STEMdiff™ Astrocyte Maturation Kit (Catalog #05850) to produce a highly pure population of astrocytes (>85% GFAP-positive astrocytes; <15% class III β-tubulin-positive neurons). Cells derived using these products are versatile tools for modeling human neurological development and disease, drug screening, toxicity testing, and cell therapy validation.
Advantages:
• Supports rapid and efficient differentiation of hPSC-derived NPCs to astrocytic precursors
• Precursors generated can be matured to functional astrocytes using the STEMdiff™ Astrocyte Maturation Kit
• Part of a system that enables generation of astrocytes from hPSCs in as few as 7 weeks; high-passage NPCs not required
• Optimized for differentiation of NPCs generated using the STEMdiff™ Neural Induction Medium embryoid body protocol
• Enables reproducible differentiation of NPCs derived from multiple human ES and iPS cell lines
Components:
  • STEMdiff™ Astrocyte Differentiation Basal Medium, 100 mL
  • STEMdiff™ Astrocyte Differentiation Supplement A, 8 mL
  • STEMdiff™ Astrocyte Differentiation Supplement B, 2 mL
  • STEMdiff™ Astrocyte Differentiation Supplement C, 100 µL
Subtype:
Specialized Media
Cell Type:
Pluripotent Stem Cells; Neural Stem and Progenitor Cells; Astrocytes; Neural Cells, PSC-Derived
Species:
Human
Application:
Cell Culture; Differentiation
Brand:
STEMdiff
Area of Interest:
Neuroscience; Stem Cell Biology; Disease Modeling; Drug Discovery and Toxicity Testing

Scientific Resources

Educational Materials

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Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Figure 1. Downstream Differentiation of Neural Progenitor Cells to Astrocytes

Neural Progenitor Cells derived from ES cells (H9 cell line) using the STEMdiff™ Neural Induction Medium embryoid body protocol were differentiated and matured to astrocytes with typical star-like morphology by culture in STEMdiff™ Astrocyte Differentiation Kit for 19-20 days and STEMdiff™ Astrocyte Maturation Kit for 56 days. The astrocytes express GFAP (green) but not the neuronal marker class III β-tubulin (red). Nuclei are counterstained with DAPI.

STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.