Human iPSC-Derived Neural Progenitor Cells

Frozen human neural progenitor cells differentiated from the human induced pluripotent stem cell line, SCTi003-A

Human iPSC-Derived Neural Progenitor Cells

Frozen human neural progenitor cells differentiated from the human induced pluripotent stem cell line, SCTi003-A

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Frozen human neural progenitor cells differentiated from the human induced pluripotent stem cell line, SCTi003-A
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Product Advantages


  • Expand immediately post-thaw with STEMdiff™ Neural Progenitor Medium

  • Save time by starting your differentiation workflow with a highly characterized neural progenitor intermediate

  • Differentiate into forebrain neurons and/or astrocytes using the STEMdiff™ neural system

  • Ensure relevance with neuron-astrocyte co-cultures generated with the same genetic background

  • Obtain high-quality NPCs, derived from the highly characterized control line, SCTi003-A

What's Included

  • Human iPSC-Derived Neural Progenitor Cells (Catalog # 200-0620)
Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.

Overview

Start your neural workflow confidently with high-quality, ready-to-use human neural progenitor cells (NPCs). These specially cryopreserved central nervous system (CNS)-type progenitors are differentiated from the human induced pluripotent stem cell (iPSC) control line, SCTi003-A, which is derived from healthy female donor peripheral blood mononuclear cells (PBMCs). Ready to use directly from thawing, these human NPCs are multipotent and suitable for customized downstream differentiation to various CNS cell types, such as forebrain neurons, midbrain neurons, and astrocytes, using STEMdiff™ kits.

To ensure high-quality cells, these NPCs were differentiated from the robust, extensively tested SCTi003-A line using serum-free STEMdiff™ SMADi Neural Induction Kit. NPCs can be expanded using STEMdiff™ Neural Progenitor Medium, allowing for scale-up and reducing the cost of workflows that require large numbers of cells. Cryopreserve expanded NPCs using STEMdiff™ Neural Progenitor Freezing Medium for flexibility in your experimental schedule.

This research-use-only (RUO) product has been consented for both academic and commercial use. Blood samples are ethically sourced using Institutional Review Board (IRB) or other regulatory authority-approved consent forms and protocols. For donor details and cell quality characterization of the source cell banks, refer to the data figures on this page. SCTi003-A is derived from an αβ T cell and has undergone VDJ sequence rearrangement. It is karyotypically stable, demonstrates trilineage differentiation potential, expresses undifferentiated cell markers, and was reprogrammed using a non-integrating reprogramming technology. Registration with hPSCreg® ensures ethical and biological conformity based on community standards. For additional details, refer to the lot-specific Certificate of Analysis and Frequently Asked Questions about iPSC lines.
Contains
STEMdiff™ Neural Progenitor Freezing Medium
Cell Type
Neural Stem and Progenitor Cells
Species
Human
Cell and Tissue Source
Pluripotent Stem Cells
Application
Cell Culture
Brand
STEMdiff
Area of Interest
Disease Modeling, Drug Discovery and Toxicity Testing, Neuroscience

Data Figures

Thawed and plated Human iPSC-Derived Neural Progenitor Cells exhibit high-quality, characteristic NPC morphology.

Figure 1. Human iPSC-Derived Neural Progenitor Cells Exhibit High-Quality Morphology Characteristic of Multipotent Central Nervous System Progenitor Cells

Cryopreserved Human iPSC-Derived Neural Progenitor Cells were thawed and plated onto Corning® Matrigel®-coated plates at 200,000 cells/cm². NPCs were incubated for 24 hours in STEMdiff™ Neural Progenitor Medium at 37℃ and subsequently analyzed by brightfield microscopy. NPCs display the small, teardrop-shaped morphology expected for NPCs. (A) 4X magnification, (B) 10X magnification. iPSC = induced pluripotent stem cell; NPCs = neural progenitor cells.

Immunocytochemistry images show Human iPSC-Derived Neural Progenitor Cells expressing neural progenitor markers SOX1 and PAX6, with low βIII-TUB expression.

Figure 2. Human iPSC-Derived Neural Progenitor Cells Express Characteristic Markers

Human iPSC-Derived Neural Progenitor Cells generated from SCTi003-A iPSCs were thawed, established in culture, and fixed for immunocytochemistry. The NPCs express neural progenitor markers (A) SOX1 and (B) PAX6 with low expression of (C) βIII-TUB. (D) In addition, they display the expected small, teardrop-shaped morphology. (E) The percentage expression of these markers were quantified. Neural progenitor markers PAX6 and SOX1 were found to be expressed in 90% of NPCs, while mature neuronal marker βIII-TUB was expressed in less than 10% of NPCs. Error bars represent standard deviation (n = 2 biological replicates). iPSC = induced pluripotent stem cell; NPCs = neural progenitor cells.

Immunocytochemistry images show Human iPSC-Derived Neural Progenitor Cells differentiated to forebrain neurons expressing neural identity marker βIII-TUB.

Figure 3. Human iPSC-Derived Neural Progenitor Cells Can Effectively Differentiate into Forebrain Neurons

Forebrain neurons were generated from Human iPSC-Derived Neural Progenitor Cells using STEMdiff™ Forebrain Neuron Differentiation Medium. NPCs were cultured for six days at 37℃ in STEMdiff™ Forebrain Neuron Differentiation Medium. Cells were subsequently cultured in STEMdiff™ Forebrain Neuron Maturation medium for 14 days. Resulting cells were processed for immunocytochemistry. (A) The resulting forebrain neuron cultures contain a population of cells expressing (B) neuronal identity marker βIII-TUB (magenta), (C) but not astrocyte marker GFAP (green). (D) Nuclei are labeled with DAPI (gray). iPSC = induced pluripotent stem cell; NPCs = neural progenitor cells.

Immunocytochemistry images show Human iPSC-Derived Neural Progenitor Cells differentiated to midbrain neurons expressing neural identity marker, βIII-TUB, and dopaminergic neuron marker, TH, but not astrocyte marker, GFAP.

Figure 4. Human iPSC-Derived Neural Progenitor Cells Can Effectively Differentiate into Midbrain Neurons

Midbrain neurons were generated from Human iPSC-Derived Neural Progenitor Cells using STEMdiff™ Midbrain Neuron Differentiation Medium. NPCs were cultured for six days at 37℃ in STEMdiff™ Midbrain Neuron Differentiation Medium. Cells were subsequently cultured in STEMdiff™ Midbrain Neuron Maturation medium for 14 days. Resulting cells were processed for immunocytochemistry. (A) The resulting midbrain neuron cultures contain a population of cells expressing (B) neuronal identity marker βIII-TUB (red) and (C) dopaminergic neuron marker TH (green), (D) but not astrocyte marker GFAP (magenta). (D) Nuclei are labeled with DAPI (blue). iPSC = induced pluripotent stem cell; NPCs = neural progenitor cells.

Immunocytochemistry images show Human iPSC-Derived Neural Progenitor Cells differentiated to astrocytes expressing astrocyte identity marker S100β and GFAP, but not neuronal marker DCX.

Figure 5. Human iPSC-Derived Neural Progenitor Cells Can Effectively Differentiate into Astrocytes

Astrocytes were generated from Human iPSC-Derived Neural Progenitor Cells using STEMdiff™ Astrocyte Differentiation Medium. NPCs were cultured for 21 days at 37℃ in STEMdiff™ Astrocyte Differentiation Medium. Cells were subsequently cultured in STEMdiff™ Astrocyte Maturation medium for seven days. Resulting cells were processed for immunocytochemistry. (A) The resulting astrocyte cultures contain a population of cells expressing (B) astrocyte marker S100β (green) and (C) GFAP (red), but not neuronal marker DCX (magenta). Nuclei are labeled with DAPI (blue). iPSC = induced pluripotent stem cell; NPCs = neural progenitor cells.

Bar graph showing expansion rate of Human iPSC-Derived Neural Progenitor Cells to 5 passages.

Figure 6. Human iPSC-Derived Neural Progenitor Cells Can Be Expanded for Multiple Passages

Human iPSC-Derived Neural Progenitor Cells were thawed and maintained in STEMdiff™ Neural Progenitor Medium at 37℃ and passaged roughly once every seven days for a total of five weeks. The average fold increase of NPCs was 2.8 ± 0.5 (mean ± SEM) per passage, demonstrating cells can be effectively expanded.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
200-0620, 200-0621
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.