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STEMdiff™ Pluripotent Stem Cell (ESC and iPSC) Differentiation

Human pluripotent stem cell (hPSC)-based models hold tremendous potential for the study of human development and disease. When working with hPSCs, even the most detailed and rigorously followed stem cell differentiation protocols can still lead to inconsistent differentiation.1,2 Use STEMdiff™ to reproducibly differentiate across multiple human embryonic stem (ES) cell and induced pluripotent stem (iPS) cell lines. STEMdiff™ is part of the most complete system of reagents for human pluripotent stem cell (hPSC) culture - a system that includes mTeSR™1 , the most published feeder-free ES cell and iPS cell culture medium. Media formulations, raw material specifications and manufacturing processes for all STEMdiff™ products have been carefully optimized in order to minimize reagent variability. In addition, STEMdiff™ products include detailed, user-friendly protocols to standardize your differentiation procedures.

Why Use STEMdiff™?

  • Reduce variability between experiments.
  • Use with hPSCs cultured with TeSR™ maintenance media.
  • Optimized formulations and pre-screened components ensure reagent consistency and minimal lot-to-lot variability.
  • Detailed protocols for differentiating to all three germ layers.
  • Efficient differentiation of multiple human ES and iPS cell lines.

Which lineages are you differentiating hPSCs to? Ectoderm: Neurons, glial cells, brain organoids, and more. Mesoderm: Hematopoietic cells, immune cells, cardiomyocytes, and more. Endoderm: Intestinal organoids, lung progenitors, and more. Customizable differentiation to multiple lineages

Explore resources for hPSC differentiation, including videos and webinars, technical tips and protocols, and more.




STEMdiff™ for ES and iPS Cell Differentiation to Endodermal Lineages

Definitive Endoderm


Lung Cells and Organoids


Pancreatic Progenitor Cells


Gastrointestinal Organoids


STEMdiff™ for ES and iPS Cell Differentiation to Multiple Lineages

For customized differentiation protocols, explore a range of unsupplemented media that supports cell survival without lineage bias. Use to direct differentiation to a variety of cell lineages, including endothelial,3 smooth muscle,3 hematopoietic,4,5 skeletal muscle,6 and cardiomyocyte7.


Assessing Pluripotency by Trilineage Differentiation

Validate the ability of new or established human ES and iPS cell lines to differentiate to the three germ layers: ectoderm, mesoderm, and endoderm.


Antibody Finder Tool

Use our Antibody Finder Tool to ensure consistent downstream cell analysis. Find antibodies that are verified to work with STEMdiff™ products in specific applications.

Find the Right Antibody for Your Applications

Don't waste valuable research time finding the appropriate antibody for your cell analysis. Use our Antibody Finder Tool to select high-quality primary and secondary antibodies verified to work with STEMdiff™ products in specific applications.

View Tool >

Find More Products for hPSC Differentiation

Cytokines
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Small Molecules
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Cultureware and General Supplies

References

  1. D’Amour KA et al. (2005) Efficient differentiation of human embryonic stem cells to definitive endoderm. Nature Biotechnology 23(12):1534-41
  2. Kattman SJ et al. (2011) Stage-Specific Optimization of Activin/Nodal and BMP Signaling Promotes Cardiac Differentiation of Mouse and Human Pluripotent Stem Cell Lines. Cell Stem Cell 8(2): 228-240
  3. Tan JY et al. (2013) Efficient Derivation of Lateral Plate and Paraxial Mesoderm Subtypes from Human Embryonic Stem Cells Through GSKi-Mediated Differentiation. Stem Cells Dev 22(13): 1893-1906
  4. Protocol adapted from Chadwick et al. (2003) and Ng et al. (2008), and discussed in this Technical Bulletin
  5. Gertow et al. (2013) WNT3A Promotes Hematopoietic or Mesenchymal Differentiation from hESCs Depending on the Time of Exposure. Stem Cell Reports 1(1):53-65
  6. Xu C et al. (2013) A Zebrafish Embryo Culture System Defines Factors that Promote Vertebrate Myogenesis across Species. 155(4): 909-921
  7. Elliott DA et al. (2011) NKX2-5(eGFP/w) hESCs for isolation of human cardiac progenitors and cardiomyocytes. Nat Methods 8(12):1037-1040
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