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Human Recombinant SCF, ACF

Stem cell factor, animal component-free

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Human Recombinant SCF, ACF

Stem cell factor, animal component-free

From: 235 USD
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Stem cell factor, animal component-free
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Overview

Stem cell factor (SCF) is an early-acting cytokine that plays a pivotal role in the regulation of embryonic and adult hematopoiesis. SCF promotes cell survival, proliferation, differentiation, adhesion, and functional activation of cells at multiple levels of the hematopoietic hierarchy. Together with other cytokines such as thrombopoietin and Flt3/Flk-2 Ligand, SCF is commonly used to promote expansion of primitive hematopoietic stem cells and multi-potent progenitor cells in culture (Martin et al.; Kent et al.). In synergy with various growth factors, including IL-2, IL-3, IL-6, IL-7, G-CSF, and erythropoietin, SCF increases proliferation and differentiation of myeloid and erythroid progenitor cells and a subset of lymphoid progenitor cells (Broudy). SCF is also a primary growth and activation factor for mast cells and eosinophils.


SCF exists in two biologically active splice forms: a soluble and a transmembrane isoform. Upon binding to its receptor (c-Kit tyrosine kinase receptor; CD117), it activates PI3K, JAK/STAT, and MAPK pathways. SCF and signaling from c-Kit have also been reported to play an important role in pigmentation, fertility, vasculogenesis, motility of the gut via c-Kit positive interstitial cells of Cajal, and in the migration of neuronal stem and progenitor cells to sites of injury in the brain. This product is animal component-free.
Subtype
Cytokines
Alternative Names
Kit ligand, Mast cell growth factor, Steel factor, Stem cell factor
Cell Type
Hematopoietic Stem and Progenitor Cells, Mesoderm, PSC-Derived, Pluripotent Stem Cells
Species
Human
Area of Interest
Stem Cell Biology
Formulation Category
Animal Component-Free
Purity
≥ 97%

Data Figures

(A) The biological activity of Human Recombinant SCF, ACF was tested by its ability to promote the proliferation of TF-1 cells. Cell proliferation was measured after 72 hours of culture using a fluorometric assay method. The EC50 is defined as the effective concentration of the cytokine at which cell proliferation is at 50% of maximum. The EC50 in the example above is 1 ng/mL.
(B) 1 μg of Human Recombinant SCF, ACF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet 1
Product Name
Human Recombinant SCF, ACF
Catalog #
78155.2, 78155.1, 78155
Lot #
1000122888 or higher
Language
English
Document Type
Product Information Sheet 2
Product Name
Human Recombinant SCF, ACF
Catalog #
78155.2, 78155.1, 78155
Lot #
1000122887 or lower
Language
English
Document Type
Safety Data Sheet
Product Name
Human Recombinant SCF, ACF
Catalog #
78155.2, 78155.1, 78155
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (10)

Cytokines, Chemokines and Growth Factors
Brochure
Cytokines, Chemokines and Growth Factors
Products for Human Pluripotent Stem Cells
Brochure
Products for Human Pluripotent Stem Cells
hPSC Differentiation: Tools for Pluripotent Stem Cell-Derived Research
Brochure
hPSC Differentiation: Tools for Pluripotent Stem Cell-Derived Research
Weekend-Free Culture of Human Pluripotent Stem Cells in mTeSR™1 or TeSR™-E8™
Technical Bulletin
Weekend-Free Culture of Human Pluripotent Stem Cells in mTeSR™1 or TeSR™-E8™
Stem Cell States: Naive to Primed Pluripotency
Wallchart
Stem Cell States: Naive to Primed Pluripotency
Identification of Colonies Derived from Human Hematopoietic Progenitors
Wallchart
Identification of Colonies Derived from Human Hematopoietic Progenitors
Directed Differentiation of Pluripotent Stem Cells
Wallchart
Directed Differentiation of Pluripotent Stem Cells
New Tools for the Ex Vivo Expansion of Human Hematopoietic Stem and Progenitor Cells
1:06:52
Webinar
New Tools for the Ex Vivo Expansion of Human Hematopoietic Stem and Progenitor Cells
Methods and Culture Reagents to Maintain a Spectrum of hPSC States from Naive-Like to Primed
23:35
Webinar
Methods and Culture Reagents to Maintain a Spectrum of hPSC States from Naive-Like to Primed
Hematopoietic Stem and Progenitor Cells (HSPCs): Isolation, Culture, and Assays
Mini Review
Hematopoietic Stem and Progenitor Cells (HSPCs): Isolation, Culture, and Assays

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Quality Statement:

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.

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