Human Recombinant G-CSF, ACF

Granulocyte colony-stimulating factor, animal component-free
Catalog #
78138_C
Granulocyte colony-stimulating factor, animal component-free
From: 204 USD

Overview

Granulocyte colony-stimulating factor (G-CSF) is a member of the CSF family of glycoproteins that regulate hematopoietic cell proliferation, differentiation, and function. It is a key cytokine involved in the production of neutrophils and the stimulation of granulocyte colony formation from hematopoietic progenitor cells (Metcalf & Nicola). G-CSF causes a range of effects including a transient reduction of SDF-1 expression (Petit et al.), the activation of metalloproteases that cleave VCAM-1 (Levesque et al.), and the release of norepinephrine from the sympathetic nervous system (Katayama et al.), leading to the release or mobilization of hematopoietic stem cells from the bone marrow into the periphery. The G-CSF receptor is expressed on a variety of hematopoietic cells, including myeloid-committed progenitor cells, neutrophils, granulocytes, and monocytes. In addition to hematopoietic cells, G-CSF is also expressed in cardiomyocytes, neuronal cells, mesothelial cells, and endothelial cells. Binding of G-CSF to its receptor leads to activation of the JAK/STAT, MAPK, PI3K, and AKT signal transduction pathways. This product is animal component-free.
Subtype
Cytokines
Alternative Names
Colony-stimulating factor 3, CSF-3, MGI-1G, Pluripoietin
Cell Type
Cancer Cells and Cell Lines, Hematopoietic Stem and Progenitor Cells, Mesoderm, PSC-Derived, Neurons
Area of Interest
Neuroscience, Stem Cell Biology
Molecular Weight
18.8 kDa
Purity
≥ 95%

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet 1
Product Name
Human Recombinant G-CSF, ACF
Catalog #
78138
Lot #
Lot BX28618 and lower
Language
English
Document Type
Product Information Sheet 2
Product Name
Human Recombinant G-CSF, ACF
Catalog #
78138
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Human Recombinant G-CSF, ACF
Catalog #
78138
Lot #
All
Language
English

Educational Materials (8)

Brochure
Cytokines, Chemokines and Growth Factors
Brochure
Small Molecules for Stem Cell Research
Brochure
Tools For Your Immunology Research
Wallchart
Identification of Colonies Derived from Human Hematopoietic Progenitors
Wallchart
Frequencies of Cell Types in Human Peripheral Blood
Video
Cell Isolation Directly from Whole Blood without RBC Lysis or Centrifugation: EasySep™ Direct
1:44
Cell Isolation Directly from Whole Blood without RBC Lysis or Centrifugation: EasySep™ Direct
Webinar
New Tools for the Ex Vivo Expansion of Human Hematopoietic Stem and Progenitor Cells
1:06:52
New Tools for the Ex Vivo Expansion of Human Hematopoietic Stem and Progenitor Cells
Mini Review
Hematopoietic Stem and Progenitor Cells (HSPCs): Isolation, Culture, and Assays

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

(A) The biological activity of Human Recombinant G-CSF, ACF was tested by its ability to promote the proliferation of mouse NFS-60 cells. Cell proliferation was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the cytokine at which cell proliferation is at 50% of maximum. The EC50 in the example above is 0.053 ng/mL. (B) 1 μg of Human Recombinant G-CSF, ACF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant G-CSF, ACF has a predicted molecular mass of 18.8 kDa.

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