Human Recombinant G-CSF, ACF

Granulocyte colony-stimulating factor, animal component-free

Human Recombinant G-CSF, ACF

Granulocyte colony-stimulating factor, animal component-free

From: 209 USD
Catalog #
78138_C
Granulocyte colony-stimulating factor, animal component-free

Overview

Granulocyte colony-stimulating factor (G-CSF) is a member of the CSF family of glycoproteins that regulate hematopoietic cell proliferation, differentiation, and function. It is a key cytokine involved in the production of neutrophils and the stimulation of granulocyte colony formation from hematopoietic progenitor cells (Metcalf & Nicola). G-CSF causes a range of effects including a transient reduction of SDF-1 expression (Petit et al.), the activation of metalloproteases that cleave VCAM-1 (Levesque et al.), and the release of norepinephrine from the sympathetic nervous system (Katayama et al.), leading to the release or mobilization of hematopoietic stem cells from the bone marrow into the periphery. The G-CSF receptor is expressed on a variety of hematopoietic cells, including myeloid-committed progenitor cells, neutrophils, granulocytes, and monocytes. In addition to hematopoietic cells, G-CSF is also expressed in cardiomyocytes, neuronal cells, mesothelial cells, and endothelial cells. Binding of G-CSF to its receptor leads to activation of the JAK/STAT, MAPK, PI3K, and AKT signal transduction pathways. This product is animal component-free.
Subtype
Cytokines
Alternative Names
Colony-stimulating factor 3, CSF-3, MGI-1G, Pluripoietin
Cell Type
Cancer Cells and Cell Lines, Hematopoietic Stem and Progenitor Cells, Mesoderm, PSC-Derived, Neurons
Species
Human
Area of Interest
Neuroscience, Stem Cell Biology
Molecular Weight
18.8 kDa
Purity
≥ 95%

Data Figures

(A) The biological activity of Human Recombinant G-CSF, ACF was tested by its ability to promote the proliferation of mouse NFS-60 cells. Cell proliferation was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the cytokine at which cell proliferation is at 50% of maximum. The EC50 in the example above is 0.053 ng/mL. (B) 1 μg of Human Recombinant G-CSF, ACF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant G-CSF, ACF has a predicted molecular mass of 18.8 kDa.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
78138
Lot #
Lot BX28618 and lower
Language
English
Catalog #
78138
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
78138
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

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