Human Recombinant Flt3/Flk-2 Ligand

Fms-like tyrosine kinase 3/fetal liver kinase-2

Human Recombinant Flt3/Flk-2 Ligand

Fms-like tyrosine kinase 3/fetal liver kinase-2

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Fms-like tyrosine kinase 3/fetal liver kinase-2
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Overview

Flt3/Flk-2 (Fms-like tyrosine kinase 3/fetal liver kinase-2) Ligand is a hematopoietic cytokine that plays an important role as a co-stimulatory factor in the proliferation, differentiation, and survival of hematopoietic stem and progenitor cells and in the development of the immune system (Hannum et al.). Flt3/Flk-2 Ligand, together with stem cell factor and thrombopoietin, is commonly used to promote expansion of primitive CD34+ hematopoietic cells in culture. In combination with myeloid cytokines such as GM-CSF, G-CSF, or M-CSF, Flt3/Flk-2 Ligand enhances the growth and numbers of clonogenic myeloid progenitor cells. In synergy with the interleukins IL-3, IL-4, IL-7, IL-11, IL-12, IL-15, and GM-CSF and TNF-α, Flt3/Flk-2 Ligand regulates the development of various lymphoid progenitor cells, including dendritic cell, B cell, T cell, and NK cell progenitors. In contrast, Flt3/Flk-2 Ligand has no significant effect on erythropoiesis or megakaryopoiesis (Drexler & Quentmeier; Wodnar-Filipowicz).

Flt3/Flk-2 Ligand exists as membrane-bound and soluble isoforms. Both isoforms are biologically active and signal through the class III tyrosine kinase receptor (Flt3/Flk-2, CD135; Rosnet et al.). Flt3/Flk-2 Ligand is produced by a variety of cell types, including uncommitted and committed hematopoietic cells and stromal fibroblasts, whereas expression of the Flt3/Flk-2 receptor is restricted to CD34+ hematopoietic stem and progenitor cells. Flt3/Flk-2 receptor is also expressed on leukemic cells and outside the hematopoietic system in the brain, placenta, and testis (Drexler & Quentmeier; Hannum et al.).
Subtype
Cytokines
Cell Type
Dendritic Cells, Hematopoietic Stem and Progenitor Cells, Mesoderm, PSC-Derived, NK Cells
Species
Human
Area of Interest
Immunology, Stem Cell Biology
Purity
≥ 95%

Data Figures

(A) The biological activity of Human Recombinant Flt3/Flk-2 Ligand was tested by its ability to promote the proliferation of OCI-AML5 cells. Cell proliferation was measured after 60 hours of culture using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the above example is 1.7 - 2.5 ng/mL.
(B) 1 μg of Human Recombinant Flt3/Flk-2 Ligand was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant Flt3/Flk-2 Ligand has a predicted molecular mass of 17.6 kDa.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
78009.1
Lot #
All
Language
English
Catalog #
78009.3, 78009, 78009.2
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
78009.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
78009.3, 78009, 78009.2
Lot #
All
Language
English

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