Flt3/Flk-2 ligand exists as membrane-bound and soluble isoforms. Both isoforms are biologically active and signal through the class III tyrosine kinase receptor (Flt3/Flk-2, CD135; Rosnet et al.). Flt3/Flk-2 ligand is produced by a variety of cell types, including uncommitted and committed hematopoietic cells and stromal fibroblasts, whereas expression of the Flt3/Flk-2 receptor is restricted to CD34+ hematopoietic stem and progenitor cells. Flt3/Flk-2 receptor is also expressed outside the hematopoietic system in the brain, placenta, and testis (Drexler & Quentmeier; Hannum et al.).
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Data and Publications
(A) The biological activity of Mouse Recombinant Flt3/Flk-2 Ligand was tested by its ability to promote the proliferation of AML5 cells. Cell
proliferation was measured after 65 hours of culture using a fluorometric assay method. The EC50 is defined as the effective
concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the above example is 1.3 - 2.0 ng/mL.
(B) 1 μg of Mouse Recombinant Flt3/Flk-2 Ligand was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Mouse Recombinant Flt3/Flk-2 Ligand has a predicted molecular mass of 18.6 kDa.