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Data and Publications
(A) The biological activity of Human Recombinant BMP-4 was tested by its ability to induce alkaline phosphatase production in ATDC-5 cells. Alkaline phosphatase production was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which alkaline phosphatase activity is at 50% of maximum. The EC50 in the above example is 3.99 ng/mL. (B) Human Recombinant BMP-4 was resolved with SDS-PAGE under reducing (+) conditions and non-reducing (-) conditions and visualized by Coomassie Blue staining. The predicted molecular mass is 13.1 kDa (monomer) or 26.2 kDa (dimer), but these migrate to an apparent molecular mass of 20 kDa or 34 kDa, respectively, due to variable glycosylation.