Human Recombinant BMP-4

Bone morphogenetic protein 4

More Views

From: 499 USD

Options

* Required Fields

Catalog # (Select a product)
Bone morphogenetic protein 4
From: 499 USD

Overview

Bone morphogenetic protein 4 (BMP-4) is a member of the highly conserved transforming growth factor β (TGF-β) superfamily. Mature BMP-4 is a disulfide-linked homodimeric protein consisting of two 116-amino-acid residue subunits, and is generated by the proteolytic removal of the signal peptide and propeptides (Xiao et al.). BMP-4 binds to type I and type II receptors on cells. This binding results in the phosphorylation of receptor 1, which in turn results in the phosphorylation of Smad proteins, which then go on to act as transcription factors (Zhang et al.). BMPs have been shown to be key regulators of embryogenesis and are known to play a role in the growth and differentiation of various cell types, including embryonic stem (ES) cells, induced pluripotent stem (iPS) cells, mesenchymal cells, epithelial cells, hematopoietic cells, and neuronal cells (Chadwick et al.; Graham et al.; Jones et al.; Lengerke et al.; Zhang et al.).
Alternative Names:
BMP-2B, BMP2B1, MCOPS6, OFC11, ZYME
Cell Type:
Endoderm, PSC-Derived; Hematopoietic Stem and Progenitor Cells; Mesoderm, PSC-Derived; Pluripotent Stem Cells
Species:
Human
Area of Interest:
Cancer Research; Epithelial Cell Biology; Stem Cell Biology
Molecular Weight:
13.1 kDa monomer; 26.2 kDa dimer
Purity:
≥ 95%

Scientific Resources

Product Documentation

Document Type
Product Name
Catalog #
Lot #
Language

Data and Publications

Data

(A) The biological activity of Human Recombinant BMP-4 was tested by its ability to induce alkaline phosphatase production in ATDC-5 cells. Alkaline phosphatase production was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which alkaline phosphatase activity is at 50% of maximum. The EC50 in the above example is 3.99 ng/mL. (B) Human Recombinant BMP-4 was resolved with SDS-PAGE under reducing (+) conditions and non-reducing (-) conditions and visualized by Coomassie Blue staining. The predicted molecular mass is 13.1 kDa (monomer) or 26.2 kDa (dimer), but these migrate to an apparent molecular mass of 20 kDa or 34 kDa, respectively, due to variable glycosylation.

STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.