RoboSep™ Buffer

Cell separation buffer
RoboSep™ Buffer

Buffer containing FBS

250 mL
Catalog # 20104
27 USD
RoboSep™ Buffer (5X Concentrate)

5X concentrate

250 mL
Catalog # 20124
90 USD
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Overview

RoboSep™ Buffer is recommended for EasySep™ cell separation protocols performed by RoboSep™ . Please note that one or two bottles of buffer are included with every purchase of a RoboSep™ Reagent Kit.
Contains
RoboSep™ Buffer (Catalog #20104)
• Dulbecco's phosphate-buffered saline (PBS)
• Fetal bovine serum (2%)
• EDTA (1 mM) in PBS

RoboSep™ Buffer (5X Concentrate; Catalog #20124)
• 5X Dulbecco's PBS
• Fetal bovine serum (10%)
• EDTA (5 mM) in PBS
Species
Human, Mouse, Rat, Non-Human Primate, Other
Brand
RoboSep
Area of Interest
Immunology

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
RoboSep™ Buffer
Catalog #
20104
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
RoboSep™ Buffer (5X Concentrate)
Catalog #
20124
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
RoboSep™ Buffer
Catalog #
20104
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
RoboSep™ Buffer (5X Concentrate)
Catalog #
20124
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
RoboSep™ Buffer (5X Concentrate)
Catalog #
20124
Lot #
All
Language
English

Educational Materials (4)

Brochure
Cell Separation Products for HLA Analysis
Brochure
Fully Automated Cell Isolation with RoboSep™
Wallchart
Human Immune Cytokines
Video
Automate Cell Isolation with the RoboSep™-S Cell Separation Instrument
1:41
Automate Cell Isolation with the RoboSep™-S Cell Separation Instrument

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Publications (1)

Cell reports 2020 may CRISPR-Cas9 Ribonucleoprotein-Mediated Genomic Editing in Mature Primary Innate Immune Cells. L. Riggan et al.

Abstract

CRISPR genome engineering has become a powerful tool to functionally investigate the complex mechanisms of immune system regulation. While decades of work have aimed to genetically reprogram innate immunity, the utility of current approaches is restricted by poor knockout efficiencies or limited specificity for mature cell lineages in vivo. Here, we describe an optimized strategy for non-viral CRISPR-Cas9 ribonucleoprotein (cRNP) genomic editing of mature primary mouse innate lymphocyte cells (ILCs) and myeloid lineage cells that results in an almost complete loss of single or double target gene expression from a single electroporation. Furthermore, we describe in vivo adoptive transfer mouse models that can be utilized to screen for gene function during viral infection using cRNP-edited naive natural killer (NK) cells and bone-marrow-derived conventional dendritic cell precursors (cDCPs). This resource will enhance target gene discovery and offer a specific and simplified approach to gene editing in the mouse innate immune system.
View All Publications

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