Human Recombinant TNF-alpha, ACF

Tumor necrosis factor-alpha, animal component-free
Catalog #
78157_C
Tumor necrosis factor-alpha, animal component-free
From: 101 USD

Overview

Tumor necrosis factor-α (TNF-α) is a pro-inflammatory cytokine that activates NF-kB, MAPK, and PI3K/AKT pathways. Activated T cells and macrophages are the primary producers of TNF-α in response to inflammation and infectious conditions. Many other cell types have been shown to produce TNF-α, among them B cells, NK cells, mast cells, neutrophils, dendritic cells, microglia, endothelial cells, smooth muscle cells, cardiomyocytes, and fibroblasts. TNF-α has cytotoxic effects on cancer cells in vitro by stimulating anti-tumor immuno- suppressive responses. TNF-α stimulates expression of E- and P-selectins, thus facilitating adhesion of neutrophils, monocytes, and memory T cells to activated platelets and endothelial cells (Zelová & Hosek). Other effects of TNF-α include vasodilatation and edema formation. This product is animal component-free.
Subtype
Cytokines
Alternative Names
Cachectin, Cachexin, Cytotoxin, DIF, Necrosin, TNF, TNF-α, TNFSF2, Tumor necrosis factor-α
Cell Type
Airway Cells, B Cells, Hematopoietic Stem and Progenitor Cells, Intestinal Cells, Neurons, NK Cells
Area of Interest
Epithelial Cell Biology, Immunology, Neuroscience, Stem Cell Biology
Molecular Weight
17.5 kDa
Purity
≥ 95%

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
Human Recombinant TNF-alpha, ACF
Catalog #
78157, 78157.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Human Recombinant TNF-alpha, ACF
Catalog #
78157, 78157.1
Lot #
All
Language
English

Educational Materials(6)

Brochure
Small Molecules for Stem Cell Research
Brochure
Cytokines, Chemokines and Growth Factors
Wallchart
Human Immune Cytokines
Wallchart
Identification of Colonies Derived from Human Hematopoietic Progenitors
Wallchart
The Identity and Properties of Mesenchymal Stem Cells
Mini Review
Hematopoietic Stem and Progenitor Cells (HSPCs): Isolation, Culture, and Assays

Data and Publications

Data

(A) The biological activity of Human Recombinant TNF-alpha, ACF was tested by its ability to inhibit growth of mouse L929 cells in the presence of actinomycin D. Inhibition of cell proliferation was measured after 48 hours of culture using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation inhibition is at 50% of maximum. The EC50 in the example above is 0.00877 ng/mL. (B) 1 μg of Human Recombinant TNF-alpha, ACF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining.

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