Human Recombinant IL-1 beta, ACF

Interleukin 1 beta, animal component-free
Catalog #
78143_C
Interleukin 1 beta, animal component-free
From: 204 USD

Overview

Interleukin 1 beta (IL-1β) is synthesized as an inactive precursor protein or pro-IL-1β. This precursor is cleaved intracellularly by Caspase 1 (IL-1β convertase) to form the active form of the protein that is later secreted (Allan et al.). IL-1β binds to IL-1 receptor and activates intracellular signaling via the MAPK or NF-kB pathway. IL-1β is released by monocytes, tissue macrophages, and dendritic cells in response to infection or injury and induces expression of acute-phase proteins. It also promotes the infiltration of inflammatory and immunocompetent cells from the circulation into the extravascular space and affected tissues, by stimulating the expression of adhesion molecules on endothelial cells. IL-1β also affects other immune cells; for example, it co-stimulates T cell functions together with antigen or mitogen. It also stimulates Th17 differentiation and B cell proliferation in an IL-6-dependent manner. This product is animal component-free.
Subtype
Cytokines
Alternative Names
Catabolin, Endogenous pyrogen, EP, Interleukin-1 beta, LAF, LEM, Leukocyte endogenous mediator, Lymphocyte-activating factor, MCF, Mononuclear cell factor
Cell Type
B Cells, Hematopoietic Stem and Progenitor Cells, T Cells, T Cells, CD4+, T Cells, CD8+
Area of Interest
Immunology, Stem Cell Biology
Molecular Weight
17.5 kDa
Purity
≥ 95%

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
Human Recombinant IL-1 beta, ACF
Catalog #
78143
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Human Recombinant IL-1 beta, ACF
Catalog #
78143
Lot #
All
Language
English

Educational Materials (5)

Brochure
Cytokines, Chemokines and Growth Factors
Brochure
Tools For Your Immunology Research
Wallchart
Frequencies of Cell Types in Human Peripheral Blood
Wallchart
Human Immune Cytokines
Video
Cell Isolation Directly from Whole Blood without RBC Lysis or Centrifugation: EasySep™ Direct
1:44
Cell Isolation Directly from Whole Blood without RBC Lysis or Centrifugation: EasySep™ Direct

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

(A) The biological activity of Human Recombinant IL-1 beta, ACF was tested by its ability to promote the proliferation of D10S cells. Cell proliferation was measured after 66 hours of culture using a fluorometric assay method. The EC50 is defined as the effective concentration of the cytokine at which cell proliferation is at 50% of maximum. The EC50 in the example above is 0.87 pg/mL. (B) 1 μg of Human Recombinant IL-1 beta, ACF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining.

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