Human Recombinant IL-1 beta, ACF

Interleukin 1 beta, animal component-free

Human Recombinant IL-1 beta, ACF

Interleukin 1 beta, animal component-free

From: 216 USD
Catalog #
78143_C
Interleukin 1 beta, animal component-free

Overview

Interleukin 1 beta (IL-1β) is synthesized as an inactive precursor protein or pro-IL-1β. This precursor is cleaved intracellularly by Caspase 1 (IL-1β convertase) to form the active form of the protein that is later secreted (Allan et al.). IL-1β binds to IL-1 receptor and activates intracellular signaling via the MAPK or NF-kB pathway. IL-1β is released by monocytes, tissue macrophages, and dendritic cells in response to infection or injury and induces expression of acute-phase proteins. It also promotes the infiltration of inflammatory and immunocompetent cells from the circulation into the extravascular space and affected tissues, by stimulating the expression of adhesion molecules on endothelial cells. IL-1β also affects other immune cells; for example, it co-stimulates T cell functions together with antigen or mitogen. It also stimulates Th17 differentiation and B cell proliferation in an IL-6-dependent manner. This product is animal component-free.
Subtype
Cytokines
Alternative Names
Catabolin, Endogenous pyrogen, EP, Interleukin-1 beta, LAF, LEM, Leukocyte endogenous mediator, Lymphocyte-activating factor, MCF, Mononuclear cell factor
Cell Type
B Cells, Hematopoietic Stem and Progenitor Cells, T Cells, T Cells, CD4+, T Cells, CD8+
Species
Human
Area of Interest
Immunology, Stem Cell Biology
Molecular Weight
17.5 kDa
Purity
≥ 95%

Data Figures

(A) The biological activity of Human Recombinant IL-1 beta, ACF was tested by its ability to promote the proliferation of D10S cells. Cell proliferation was measured after 66 hours of culture using a fluorometric assay method. The EC50 is defined as the effective concentration of the cytokine at which cell proliferation is at 50% of maximum. The EC50 in the example above is 0.87 pg/mL. (B) 1 μg of Human Recombinant IL-1 beta, ACF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
78143, 78143.1, 78143.2
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
78143, 78143.1, 78143.2
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Contact STEMCELL Technologies

Our Customer Service, Sales, and Product and Scientific Support departments in North America are available between 6 am and 5 pm Pacific Time (9 am and 8 pm Eastern Time). One of our representatives will be happy to help you by telephone or email. Please complete the form to contact us by email. A representative will get back to you shortly.
  •  

StemCell Technologies Inc. and affiliates ("STEMCELL Technologies") does not share your email address with third parties. StemCell Technologies Inc. will use your email address to confirm your identity and send you newsletters, transaction-related emails, promotional and customer service emails in accordance with our privacy policy. You can change your email preferences at any time.