Human Recombinant IL-2, ACF

Interleukin 2, animal component-free

Human Recombinant IL-2, ACF

Interleukin 2, animal component-free

From: 92 USD
Catalog #
78145_C
Interleukin 2, animal component-free

Overview

Interleukin 2 (IL-2) is a monomeric cytokine that was originally identified as a T cell growth factor (Gaffen & Liu). It binds to heterotrimeric receptors consisting of CD25, CD122, and CD132. Upon binding, it activates JAK3-, STAT5-, and AKT-dependent signaling pathways, which results in cellular proliferation and survival (Ma et al.). The majority of IL-2 is secreted by activated CD4+ and CD8+ T cells, although B cells and dendritic cells were found to produce IL-2 in small amounts. IL-2 downregulates immune responses to prevent autoimmunity during thymic development, influences the development of CD4+CD25+ regulatory T cells, and affects development of follicular helper T cells. IL-2 also controls inflammation by inhibiting Th17 differentiation (Banchereau et al.). High IL-2 levels in serum are associated with progression of scleroderma, rheumatoid arthritis, and gastric and non-small cell lung cancer, though no known disease can be directly attributed to the lack or excess of IL-2 (Gaffen & Liu).
Subtype
Cytokines
Alternative Names
Aldesleukin, Interleukin-2, T cell growth factor, TCGF
Cell Type
Hematopoietic Stem and Progenitor Cells, NK Cells, T Cells, T Cells, CD4+, T Cells, CD8+
Species
Human
Area of Interest
Immunology, Stem Cell Biology
Molecular Weight
15.5 kDa
Purity
≥ 95%

Data Figures

(A) The biological activity of Human Recombinant IL-2, ACF was tested by its ability to promote the proliferation of CTLL-2 cells. The EC50 is defined as the effective concentration of the cytokine at which cell proliferation is at 50% of maximum. The EC50 in the example above is 1.99 ng/mL. (B) 1 μg of Human Recombinant IL-2, ACF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
78145, 78145.1, 78145.2
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
78145, 78145.1, 78145.2
Lot #
All
Language
English

Contact STEMCELL Technologies

Our Customer Service, Sales, and Product and Scientific Support departments in North America are available between 6 am and 5 pm Pacific Time (9 am and 8 pm Eastern Time). One of our representatives will be happy to help you by telephone or email. Please complete the form to contact us by email. A representative will get back to you shortly.
  •  

StemCell Technologies Inc. and affiliates ("STEMCELL Technologies") does not share your email address with third parties. StemCell Technologies Inc. will use your email address to confirm your identity and send you newsletters, transaction-related emails, promotional and customer service emails in accordance with our privacy policy. You can change your email preferences at any time.