Human Recombinant IL-4, ACF

Interleukin 4, animal component-free
Catalog #
78147_C
Interleukin 4, animal component-free
From: 204 USD

Overview

Human Interleukin 4 (IL-4) is important for immune responses to helminth infection as well as in allergic responses (Olpihant et al.). The IL-4 receptor consists of a heterodimer of IL-4Ra and common gamma chain. IL-4 receptor engagement leads to the activation of JAK1/3 and the recruitment of STAT6 and IRS1/2 (Nelms et al.). IL-4 drives immunoglobulin class switching in B cells (to IgE, IgG4), mast cell hyperplasia, mucus production, and the differentiation of naïve T cells into T helper type 2 (Th2) cells, which produce IL-4, IL-5, IL-10, and IL-13 (Bao et al.; Olpihant et al.; Nelms et al.). In addition to Th2 T cells, IL-4 is produced by CD4+ NK T cells, γ/δ T cells, activated basophils, eosinophils, and mast cells. IL-4 consists of 130 amino acids with a predicted molecular weight of 15.1 kDa. Human IL-4 does not cross-react with mouse cells (Park et al.). This product is animal component-free.
Subtype
Cytokines
Alternative Names
B cell stimulating factor, BCDF, BCGF, BSF-1, Interleukin-4
Cell Type
B Cells, Dendritic Cells, Hematopoietic Stem and Progenitor Cells, Lymphocytes, Monocytes, Myeloid Cells, Neurons, T Cells
Area of Interest
Immunology, Neuroscience, Stem Cell Biology
Molecular Weight
15.1 kDa
Purity
≥ 95%

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
Human Recombinant IL-4, ACF
Catalog #
78147, 78147.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Human Recombinant IL-4, ACF
Catalog #
78147, 78147.1
Lot #
All
Language
English

Educational Materials (5)

Brochure
Tools For Your Immunology Research
Brochure
Cytokines, Chemokines and Growth Factors
Wallchart
Human Immune Cytokines
Wallchart
Frequencies of Cell Types in Human Peripheral Blood
Video
Cell Isolation Directly from Whole Blood without RBC Lysis or Centrifugation: EasySep™ Direct
1:44
Cell Isolation Directly from Whole Blood without RBC Lysis or Centrifugation: EasySep™ Direct

Data and Publications

Data

(A) The biological activity of Human Recombinant IL-4, ACF was tested by its ability to promote the proliferation of TF-1 cells. Cell proliferation was measured after 72 hours of culture using a fluorometric assay method. The EC50 is defined as the effective concentration of the cytokine at which cell proliferation is at 50% of maximum. The EC50 in the example above is 0.2 ng/mL. (B) 1 μg of Human Recombinant IL-4, ACF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining.

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