Human Recombinant TNF-alpha

Tumor necrosis factor alpha
Catalog #
78068_C
Tumor necrosis factor alpha
From: 85 USD

Overview

Tumor necrosis factor-α (TNF-α) is a pro-inflammatory cytokine that activates NF-kB, MAPK, and PI3K/AKT pathways. Activated T cells and macrophages are the primary producers of TNF-α in response to inflammation and infectious conditions. Many other cell types have been shown to produce TNF-α, among them B cells, NK cells, mast cells, neutrophils, dendritic cells, microglia, endothelial cells, smooth muscle cells, cardiomyocytes, and fibroblasts. TNF-α has cytotoxic effects on cancer cells in vitro by stimulating anti-tumor immuno- suppressive responses. TNF-α stimulates expression of E- and P-selectins, thus facilitating adhesion of neutrophils, monocytes, and memory T cells to activated platelets and endothelial cells (Zelová & Hosek). Other effects of TNF-α include vasodilatation and edema formation.
Subtype
Cytokines
Alternative Names
Cachectin, Cachexin, Cytotoxin, DIF, Necrosin, TNF, TNF-α, TNFSF2, Tumor necrosis factor-α
Cell Type
Airway Cells, B Cells, Hematopoietic Stem and Progenitor Cells, Intestinal Cells, Neurons, NK Cells
Species
Human
Area of Interest
Epithelial Cell Biology, Immunology, Neuroscience, Stem Cell Biology
Molecular Weight
17.5 kDa
Purity
≥ 95%

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
Human Recombinant TNF-alpha
Catalog #
78068, 78068.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Human Recombinant TNF-alpha
Catalog #
78068, 78068.1
Lot #
All
Language
English

Educational Materials (3)

Brochure
Cytokines, Chemokines and Growth Factors
Brochure
Tools For Your Immunology Research
Wallchart
Human Immune Cytokines

Data and Publications

Data

(A) The biological activity of Human Recombinant TNF-alpha was tested by its ability to induce cytolysis of L929 cells growing in the presence of actinomycin D. Cell viability was measured after 48 hours of culture using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell viability is at 50% of maximum. The EC50 in the above example is 0.4 0.6 ng/mL.
(B) 1 μg of Human Recombinant TNF-alpha was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant TNF-alpha has a predicted molecular mass of 17.5 kDa.

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