STEMdiff™ Ventral Forebrain Organoid Differentiation Kit

Cell culture medium kit for robust generation of ventral forebrain neural organoids from human pluripotent stem cells
STEMdiff™ Ventral Forebrain Organoid Differentiation Kit

Cell culture medium kit for robust generation of ventral forebrain neural organoids from human pluripotent stem cells

1 Kit
Catalog # 08630
612 USD
Required Products
  1. AggreWell™800 24-well plate|34811
    AggreWell™800

    Microwell culture plates for easy and reproducible production of embryoid bodies and spheroids

  2. AggreWell™ Rinsing Solution
    Anti-Adherence Rinsing Solution

    Rinsing solution for cultureware to prevent cell adhesion

  3. 37μm Reversible Strainers|27215,27250
    Reversible Strainers

    Reversible strainer for filtration of single cells and isolation of cellular aggregates, including embryoid bodies and spheroids

  4. STEMdiff™ Neural Organoid Maintenance Kit
    STEMdiff™ Neural Organoid Maintenance Kit

    Cell culture medium kit for long-term maintenance and maturation of patterned neural organoids

Overview

Robustly generate three-dimensional, patterned brain organoid cultures from human pluripotent stem cells without matrix embedding. STEMdiff™ Dorsal and Ventral Forebrain Organoid Differentiation Kits are serum-free cell culture media that work with AggreWell™-generated embryoid bodies to prevent organoid fusion and enable the scalable generation of over 500 highly reproducible organoids per kit. Adapted from protocols by Sergiu Paşca (F Birey et al. Nature, 2017), these brain-region-specific organoids are three-dimensional in vitro models with a cellular composition and structural organization that is representative of the developing human forebrain. STEMdiff™ Dorsal Forebrain Organoid Differentiation Kit (Catalog #08620) generates tissue of the early developing dorsal pallium, while STEMdiff™ Ventral Forebrain Organoid Differentiation Kit (Catalog #08630) generates tissue of the early developing ventral subpallium. Organoids generated with these kits can also be co-cultured as assembloids to study brain region interactions (F Birey et al. Nature, 2017). For extended periods of organoid culture (> 50 days), the components required for organoid maintenance are available as STEMdiff™ Neural Organoid Maintenance Kit (Catalog #100-0120).
Advantages
⦁ Reduce handling and media waste with fusion-free growth media
⦁ Obtain greater analytic sensitivity for disease phenotypes with reproducible morphology between lines and individual organoids
⦁ Generate over 500 organoids per kit using AggreWell™800 for higher-powered statistical replicates and more detailed longitudinal study
⦁ Eliminate matrix embedding steps with the matrix-free formulation and protocol
⦁ Easily process and section consistently sized organoids
⦁ Achieve long-term culture survival and reduced caspase-3 expression for neurotoxicity and neurodegenerative models
⦁ Combine modular region-patterned organoids to generate advanced assembloids for disease modeling and regenerative applications
Components
  • STEMdiff™ Neural Organoid Basal Medium 1, 20 mL
  • STEMdiff™ Neural Organoid Basal Medium 2, 500 mL
  • STEMdiff™ Neural Organoid Supplement A, 10 mL
  • STEMdiff™ Neural Organoid Supplement B, 0.25 mL
  • STEMdiff™ Neural Organoid Supplement C, 0.25 mL
  • STEMdiff™ Neural Organoid Supplement D, 0.5 mL
Subtype
Specialized Media
Cell Type
Neural Cells, PSC-Derived, Neural Stem and Progenitor Cells, Pluripotent Stem Cells
Species
Human
Application
Cell Culture, Characterization, Differentiation, Functional Assay, Immunofluorescence, Organoid Culture, Phenotyping, Spheroid Culture
Brand
STEMdiff
Area of Interest
Disease Modeling, Drug Discovery and Toxicity Testing, Neuroscience, Organoids
Formulation
Serum-Free

Related Products

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
STEMdiff™ Ventral Forebrain Organoid Differentiation Kit
Catalog #
08630
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
STEMdiff™ Ventral Forebrain Organoid Differentiation Kit
Catalog #
08630
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
STEMdiff™ Ventral Forebrain Organoid Differentiation Kit
Catalog #
08630
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
STEMdiff™ Ventral Forebrain Organoid Differentiation Kit
Catalog #
08630
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
STEMdiff™ Ventral Forebrain Organoid Differentiation Kit
Catalog #
08630
Lot #
All
Language
English
Document Type
Safety Data Sheet 5
Product Name
STEMdiff™ Ventral Forebrain Organoid Differentiation Kit
Catalog #
08630
Lot #
All
Language
English
Document Type
Safety Data Sheet 6
Product Name
STEMdiff™ Ventral Forebrain Organoid Differentiation Kit
Catalog #
08630
Lot #
All
Language
English

Educational Materials(14)

Brochure
STEMCELL Neural Product Portfolio
Brochure
hPSC-Derived Neural Cell Research
Brochure
Organoids
Technical Bulletin
Cryogenic Tissue Processing and Section Immunofluorescence of Cerebral Organoids
Wallchart
Derivation and Applications of Human Pluripotent Stem Cells
Wallchart
Cell-Reprogramming Technology and Neuroscience
Wallchart
Building Three-Dimensional Human Brain Organoids
Webinar
1:02:03
Building Brain Organoids and Assembloids to Study Human Development and Disease
Webinar
41:47
Challenges in Translating iPSC Technology
Webinar
17:17
Nature Research Round Table: The Promise of Organoid Medicine
Webinar
50:49
hPSC Quality: Essential Considerations for Gene Editing, Cloning, Maintenance and Disease Modeling
Webinar
1:01:37
ISSCR Innovation Showcase: Advanced Brain Organoid Co-Culture Systems
Webinar
Spotlight on Organoids: Expert Panel
Scientific Poster
A Reliable, Efficient, and Feeder-Free Method to Generate Brain-Region-Specific Dorsal and Ventral Forebrain Organoids From Human Pluripotent Stem Cells to Model Early Human Brain Development
Load More Educational Materials

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Schematic protocol diagram to generate patterned ventral forebrain organoids from iPSCs

Figure 1. Schematic for the STEMdiff™ Ventral Forebrain Organoid Differentiation Kit

Human ES or iPS cell-derived ventral forebrain organoids can be generated in 43 days. Embryoid bodies can be created in 6 days with AggreWell™800 plates. The EBs are then cultured in suspension, allowing growth and subsequent patterning to the ventral forebrain. For the long-term maintenance and further maturation of ventral forebrain organoids, see the PIS.

Figure 2. Fluorescent Imaging in BrainPhys™ Imaging Optimized Medium Improves Signal-to-Background Ratios of 3D Neural Cultures

GFP-labeled ventral forebrain organoids were co-cultured and fused with unlabeled dorsal forebrain organoids for one week prior to live imaging in Forebrain Organoid Differentiation Medium from STEMdiff™ Dorsal Forebrain Organoid Differentiation Kit (right) or BrainPhys™ Imaging Optimized Medium (BPI, left). Interneuron migration can be visualized more clearly in BPI. Adapted from Zabolocki et al. 2020, Nature Communications, available under a Creative Commons 4.0 License.

Contact STEMCELL Technologies

Our Customer Service, Sales, and Product and Scientific Support departments in North America are available between 6 am and 5 pm Pacific Time (9 am and 8 pm Eastern Time). One of our representatives will be happy to help you by telephone or email. Please complete the form to contact us by email. A representative will get back to you shortly.
  •  

StemCell Technologies Inc. and affiliates ("STEMCELL Technologies") does not share your email address with third parties. StemCell Technologies Inc. will use your email address to confirm your identity and send you newsletters, transaction-related emails, promotional and customer service emails in accordance with our privacy policy. You can change your email preferences at any time.