STEMdiff™ Neural Organoid Maintenance Kit

Cell culture medium kit for long-term maintenance and maturation of patterned neural organoids
STEMdiff™ Neural Organoid Maintenance Kit

Cell culture medium kit for long-term maintenance and maturation of patterned neural organoids

1 Kit
Catalog # 100-0120
276 USD

Overview

Culture patterned brain organoids for extended periods (> 50 days) without the use of embedding matrices. With the essential components to prevent organoid fusion and retain a high yield of reproducible organoids, STEMdiff™ Neural Organoid Maintenance Kit is a serum-free cell culture medium designed for the maintenance and maturation of neural organoids adapted from protocols by Sergiu Paşca (F Birey et al. Nature, 2017). When used following STEMdiff™ Dorsal or Ventral Forebrain Organoid Differentiation Kits (Catalog #08620 and #08630), STEMdiff™ Neural Organoid Maintenance Kit allows for long-term organoid experiments for neurodegeneration research, biomarker discovery, and toxicity assays. The resulting brain-region-specific organoids have a cellular composition and structural organization that is representative of the developing human forebrain. Organoids generated with these kits can also be co-cultured as assembloids to study brain region interactions (F Birey et al. Nature, 2017).
Advantages
⦁ Reduce handling and media waste with fusion-free growth media
⦁ Obtain greater analytic sensitivity for disease phenotypes with reproducible morphology between lines and individual organoids
⦁ Eliminate matrix embedding steps with the matrix-free formulation and protocol
⦁ Easily process and section consistently sized organoids
⦁ Achieve long-term culture survival and reduced caspase-3 expression for neurotoxicity and neurodegenerative models
⦁ Combine modular region-patterned organoids to generate advanced assembloids for disease modeling and regenerative applications
Components
  • STEMdiff™ Neural Organoid Basal Medium 2, 500 mL
  • STEMdiff™ Neural Organoid Supplement A, 10 mL
Subtype
Specialized Media
Cell Type
Neural Cells, PSC-Derived, Neural Stem and Progenitor Cells, Pluripotent Stem Cells
Species
Human
Application
Characterization, Differentiation, Functional Assay, Immunofluorescence, Maintenance, Organoid Culture, Phenotyping, Spheroid Culture
Brand
STEMdiff
Area of Interest
Disease Modeling, Drug Discovery and Toxicity Testing, Neuroscience, Organoids
Formulation
Serum-Free

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
STEMdiff™ Neural Organoid Maintenance Kit
Catalog #
100-0120
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
STEMdiff™ Neural Organoid Maintenance Kit
Catalog #
100-0120
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
STEMdiff™ Neural Organoid Maintenance Kit
Catalog #
100-0120
Lot #
All
Language
English

Educational Materials (15)

Brochure
STEMCELL Neural Product Portfolio
Brochure
hPSC-Derived Neural Cell Research
Brochure
Organoids
Technical Bulletin
Cryogenic Tissue Processing and Section Immunofluorescence of Cerebral Organoids
Protocol
How to Dissociate 3D Neural Organoids into a Single-Cell Suspension
Wallchart
Derivation and Applications of Human Pluripotent Stem Cells
Wallchart
Cell-Reprogramming Technology and Neuroscience
Wallchart
Building Three-Dimensional Human Brain Organoids
Webinar
Building Brain Organoids and AssemBloids™ to Study Human Development and Disease
1:02:03
Building Brain Organoids and AssemBloids™ to Study Human Development and Disease
Webinar
Challenges in Translating iPSC Technology
41:47
Challenges in Translating iPSC Technology
Webinar
Nature Research Round Table: The Promise of Organoid Medicine
17:17
Nature Research Round Table: The Promise of Organoid Medicine
Webinar
hPSC Quality: Essential Considerations for Gene Editing, Cloning, Maintenance and Disease Modeling
50:49
hPSC Quality: Essential Considerations for Gene Editing, Cloning, Maintenance and Disease Modeling
Webinar
ISSCR Innovation Showcase: Advanced Brain Organoid Co-Culture Systems
1:01:37
ISSCR Innovation Showcase: Advanced Brain Organoid Co-Culture Systems
Webinar
Spotlight on Organoids: Expert Panel
Scientific Poster
A Reliable, Efficient, and Feeder-Free Method to Generate Brain-Region-Specific Dorsal and Ventral Forebrain Organoids From Human Pluripotent Stem Cells to Model Early Human Brain Development
Load More Educational Materials

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

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