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Data

Figure 1. Data for Alexa Fluor® 488-Conjugated
Flow cytometry analysis of human neural progenitor cells (NPCs) generated from induced pluripotent stem (iPS) cells using STEMdiff™ Neural Induction Medium and cultured on Corning® Matrigel®. NPCs were fixed and labeled with Anti-Human Nestin Antibody, Clone 10C2, Alexa Fluor® 488 (filled histogram) or a mouse IgG1, kappa Alexa Fluor® 488 isotype control antibody (solid line histogram).

Figure 2. Data for Unconjugated
(A) Human neural progenitor cells (NPCs) were generated from induced pluripotent stem (iPS) cells using STEMdiff™ Neural Induction Medium and cultured on Corning® Matrigel®-coated glass coverslips. Cells were fixed and labeled with Anti-Human Nestin Antibody, Clone 10C2, followed by donkey anti-mouse IgG, Alexa Fluor® 488. Inset shows cells incubated with a mouse IgG1, kappa isotype control antibody followed by donkey anti-mouse IgG, Alexa Fluor® 488.
(B) Flow cytometry analysis of human NPCs generated from iPS cells using STEMdiff™ Neural Induction Medium and cultured on Corning® Matrigel®. NPCs were fixed and labeled with Anti-Human Nestin Antibody, Clone 10C2, followed by goat anti-mouse IgG, FITC (filled histogram) or a mouse IgG1, kappa isotype control antibody followed by goat anti-mouse IgG, FITC (solid line histogram).
(C) Western blot analysis of human umbilical vein endothelial cell (HUVEC) lysate with Anti-Human Nestin Antibody, Clone 10C2.