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Anti-Human Nestin Antibody, Clone 10C2

Mouse monoclonal IgG1 antibody against human, cynomolgus nestin

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Anti-Human Nestin Antibody, Clone 10C2

Mouse monoclonal IgG1 antibody against human, cynomolgus nestin

From: 179 USD
Catalog #
60091_C
Mouse monoclonal IgG1 antibody against human, cynomolgus nestin
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Overview

The 10C2 antibody reacts with human nestin, a class VI intermediate filament (IF) protein expressed by embryonic neuroepithelial stem cells in the developing nervous system and by pancreatic islet and mesenchymal progenitor cells. Nestin expression is also observed in glioblastomas and many primary and metastatic melanomas and it is a useful angiogenic marker to evaluate neovascularity of endothelial cells in tumors. Nestin is required for the survival and renewal of neural progenitor cells and plays roles in cellular remodeling by forming heterodimers with vimentin or alpha-internexin, a process that appears to be tightly regulated by phosphorylation. Nestin localizes to the growth cones during axon elongation in differentiating neurons and may play a role in growth cone guidance. Nestin expression is down-regulated during differentiation, typically disappearing by embryonic stage E18. The 10C2 antibody reportedly cross-reacts weakly with monkey nestin but not rodent nestin. A doublet band of ~220 - 250 kDa is observed on western blots with this antibody.
Subtype
Primary Antibodies
Target Antigen
Nestin
Alternative Names
NES, type VI intermediate filament (IF) protein
Reactive Species
Cynomolgus, Human
Conjugation
Alexa Fluor 488, Unconjugated
Host Species
Mouse
Cell Type
Neural Cells, PSC-Derived, Neural Stem and Progenitor Cells, Neurons
Species
Human, Non-Human Primate
Application
Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blotting
Area of Interest
Neuroscience, Stem Cell Biology
Clone
10C2
Gene ID
10763
Isotype
IgG1, kappa

Data Figures

Data for Alexa Fluor® 488-Conjugated

Figure 1. Data for Alexa Fluor® 488-Conjugated

Flow cytometry analysis of human neural progenitor cells (NPCs) generated from induced pluripotent stem (iPS) cells using STEMdiff™ Neural Induction Medium and cultured on Corning® Matrigel®. NPCs were fixed and labeled with Anti-Human Nestin Antibody, Clone 10C2, Alexa Fluor® 488 (filled histogram) or a mouse IgG1, kappa Alexa Fluor® 488 isotype control antibody (solid line histogram).

Data for Unconjugated

Figure 2. Data for Unconjugated

(A) Human neural progenitor cells (NPCs) were generated from induced pluripotent stem (iPS) cells using STEMdiff™ Neural Induction Medium and cultured on Corning® Matrigel®-coated glass coverslips. Cells were fixed and labeled with Anti-Human Nestin Antibody, Clone 10C2, followed by donkey anti-mouse IgG, Alexa Fluor® 488. Inset shows cells incubated with a mouse IgG1, kappa isotype control antibody followed by donkey anti-mouse IgG, Alexa Fluor® 488.
(B) Flow cytometry analysis of human NPCs generated from iPS cells using STEMdiff™ Neural Induction Medium and cultured on Corning® Matrigel®. NPCs were fixed and labeled with Anti-Human Nestin Antibody, Clone 10C2, followed by goat anti-mouse IgG, FITC (filled histogram) or a mouse IgG1, kappa isotype control antibody followed by goat anti-mouse IgG, FITC (solid line histogram).
(C) Western blot analysis of human umbilical vein endothelial cell (HUVEC) lysate with Anti-Human Nestin Antibody, Clone 10C2.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Anti-Human Nestin Antibody, Clone 10C2, Alexa Fluor® 488
Catalog #
60091AD, 60091AD.1
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
Anti-Human Nestin Antibody, Clone 10C2
Catalog #
60091.1, 60091
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human Nestin Antibody, Clone 10C2, Alexa Fluor® 488
Catalog #
60091AD, 60091AD.1
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Anti-Human Nestin Antibody, Clone 10C2
Catalog #
60091.1, 60091
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

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Legal Statement:

Alexa Fluor is a registered trademark of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

Quality Statement:

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.

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