Anti-Human Nestin Antibody, Clone 10C2
Mouse monoclonal IgG1 antibody against human, cynomolgus nestin
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Overview
Data Figures
Figure 1. Data for Alexa Fluor® 488-Conjugated
Flow cytometry analysis of human neural progenitor cells (NPCs) generated from induced pluripotent stem (iPS) cells using STEMdiff™ Neural Induction Medium and cultured on Corning® Matrigel®. NPCs were fixed and labeled with Anti-Human Nestin Antibody, Clone 10C2, Alexa Fluor® 488 (filled histogram) or a mouse IgG1, kappa Alexa Fluor® 488 isotype control antibody (solid line histogram).
Figure 2. Data for Unconjugated
(A) Human neural progenitor cells (NPCs) were generated from induced pluripotent stem (iPS) cells using STEMdiff™ Neural Induction Medium and cultured on Corning® Matrigel®-coated glass coverslips. Cells were fixed and labeled with Anti-Human Nestin Antibody, Clone 10C2, followed by donkey anti-mouse IgG, Alexa Fluor® 488. Inset shows cells incubated with a mouse IgG1, kappa isotype control antibody followed by donkey anti-mouse IgG, Alexa Fluor® 488.
(B) Flow cytometry analysis of human NPCs generated from iPS cells using STEMdiff™ Neural Induction Medium and cultured on Corning® Matrigel®. NPCs were fixed and labeled with Anti-Human Nestin Antibody, Clone 10C2, followed by goat anti-mouse IgG, FITC (filled histogram) or a mouse IgG1, kappa isotype control antibody followed by goat anti-mouse IgG, FITC (solid line histogram).
(C) Western blot analysis of human umbilical vein endothelial cell (HUVEC) lysate with Anti-Human Nestin Antibody, Clone 10C2.
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Resources and Publications
Educational Materials (3)
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Legal Statement:
Alexa Fluor is a registered trademark of Life Technologies Corporation. Antibodies conjugated to Alexa Fluor® are licensed for internal research use only and sale is expressly conditioned on the buyer not using the antibody for manufacturing, performing a service or medical test, or otherwise generating revenue. For use other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.
Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
