Pluripotent Stem Cell Training
Training to support the culture of hPSCs and their differentiation towards cerebral organoids, intestinal organoids, cardiomyocytes, or hematopoietic progenitors
Note on Hands-On Training Courses: STEMCELL Technologies is not currently offering this standardized in-person training course, but may again in the future. Please reach out to your representative or LiveChat agent to enquire about customized training, or contact us through our phone or email channels. In the meantime, see what Live Virtual or On-Demand courses we offer, or visit this page to learn more about our Proficiency Testing Programs.
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Please proceed to check out after completing this form. Payment information will be used to secure your registration and you will only be charged when the Intestinal Organoid Starter Kit is shipped.
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In-lab demonstration
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Overview
Speakers
Speakers will vary depending on course topic, date, and location.
Arwen Hunter, PhD
Senior Scientist (Pluripotent Group), Research and Development
STEMCELL Technologies
STEMCELL Technologies
Leon Chew, MSc
Scientist (Neural Group), Research and Development
STEMCELL Technologies
STEMCELL Technologies
Vincenzo Macri, PhD
Senior Scientist (Myogenic Group), Research and Development
STEMCELL Technologies
STEMCELL Technologies
Wing Chang, PhD
Director, Research and Development UK
STEMCELL Technologies
STEMCELL Technologies
Phillip Kramer, PhD
Senior Scientist (Intestinal Group), Research and Development
STEMCELL Technologies
STEMCELL Technologies
Agenda
Note that each day will end at approximately 4:30 pm with the course dinner taking place on the evening of Maintaining Human Pluripotent Stem Cells (hPSCs)
All course options followed by wrap-up which includes a quiz, evaluation, and certificate presentation.
Reprogramming of Somatic Cells to Induced Pluripotent Stem Cells (iPS Cells)
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Lecture: Isolation and Reprogramming of Somatic Cells to iPS Cells
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Practical Session: Isolation of Peripheral Blood Mononuclear Cells (PBMCs)
- Overview of EasySep™ cell separation system
- Demonstrate preparation of PBMCs using SepMate™
- Observe culture-expanded blood cells in StemSpan™ SFEM
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Practical Session: Reprogramming of Somatic Cells to iPS Cells
- Demonstrate transfection of human fibroblasts using ReproRNA™-OKSGM
- Observe reprogrammed cells cultured in ReproTeSR™
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Practical Session: Selection and Picking of iPS Cell Colonies
Maintaining Human Pluripotent Stem Cells (hPSCs)
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Lecture: Maintaining and Assessing High-Quality hPSC Cultures
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Lecture: Genome Editing with CRISPR-Cas9
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Practical Session: Evaluating Quality of hPSC Cultures and Plate Coating
- Observe time course of hPSC cultures and review colony morphology
- Practice plate coating with Matrigel® or Vitronectin XF™
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Practical Session: Passaging and Long-Term Maintenance of hPSCs
- Practice enzyme-free passaging of hPSCs using Gentle Cell Dissociation Reagent or ReLeSR™
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Practical Session: Cryopreservation and Thawing of hPSCs
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Dinner (Courtesy of STEMCELL Technologies)
Differentiating hPSCs (Select Option in Sign-Up Form)
Guest Lecture by Christine Mummery: Microphysiological Systems Based on hiPSC to Study Cardiovascular Disease (Available in The Netherlands session only)
Option A: Differentiation towards Cardiomyocytes (Available in Cambridge, UK and Vancouver, CA only)
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Lecture: Modeling Long QT Type 2 Syndrome using the STEMdiff™ Cardiomyocyte System
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Practical Session: Evaluation of the Quality of hPSC Cultures after Passaging
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Practical Session: Preparation of hPSCs as Single Cells for Cardiomyocyte Differentiation
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Practical Session: Observation of hPSC-Derived Cardiomyocytes
- Observe time course of hPSC-derived cardiomyocytes
- Dissociation and replating of hPSC-derived cardiomyocytes
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Practical Session: Cryopreservation and Thawing of hPSC-Derived Cardiomyocytes and Downstream Applications
Option B: Differentiation towards Hematopoietic Progenitor Cells (HPCs)
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Lecture: Differentiation of hPSCs towards Hematopoietic Progenitor Cells
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Practical Session: Evaluation of the Quality of hPSC Cultures after Passaging
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Practical Session: Observation of hPSC-Derived HPCs
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Practical Session: HPCs for Downstream Applications
- Harvest HPCs from culture supernatant
- Analyze hPSC-derived HPCs using the colony-forming unit (CFU) assay
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Practical Session: Identification and Counting of Colonies in the CFU Assay
Option C: Differentiation towards Neural Cell Types Including Cerebral Organoids
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Lecture: Differentiation of hPSCs Towards Neural Progenitors and Neural Cell Types
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Practical Session: Generation of Embryoid Bodies (EBs) Using AggreWell™ and Preparation of Single Cell hPSCs for Monolayer-Based Protocols
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Practical Session: Differentiation of hPSCs to NPCs
- Examine neural aggregates in AggreWell™ and harvest EBs
- Assess neural induction by scoring neural rosette formation
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Lecture: Using Standardized Reagents to Generate Cerebral Organoids from hPSCs
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Practical Session: Embed EBs in Matrigel® to Generate Cerebral Organoids
Option D: Differentiation towards Intestinal Organoids (Available in Cambridge, UK and Vancouver, CA only)
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Lecture: Differentiation of hPSCs Towards Intestinal Organoids
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Practical Session: Evaluate the Quality of hPSC Cultures after Passaging from Previous Day
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Lecture: Applications of hPSC-derived Intestinal Organoids
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Practical Session: Differentiation of hPSC Towards Intestinal Organoids
- Observe time course of hPSC cultures to evaluate differentiation efficiency
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Practical Session: Harvest and Embed hPSC-Derived Mid-/Hind-gut Spheroids
- Embed spheroids in Matrigel®
- Observe hPSC-derived intestinal organoids at passage 0
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Practical Session: Passage hPSC-Derived Intestinal Organoids
- Observe hPSC-derived intestinal organoids
- Passage and maintain hPSC-derived intestinal organoids
Accommodation
Vancouver, CA
Pan Pacific Vancouver
300 - 999 Canada Place, Vancouver, BC V6C 3B5
Sheraton Wall Centre
1088 Burrard St, Vancouver, BC V6Z 2R9
Executive Hotel Le Soleil
567 Hornby St, Vancouver, BC V6C 2E8
Cambridge, UK (Transport included from this location to and from training venue - Cambridge UK only)
University Arms Parker's Tavern
Regent Street Cambridge CB2 1AD
Leiden, NL
Holiday Inn Leiden
Haagse Schouwweg 10, 2332 KG, Leiden, Netherlands
IBIS Leiden Centre
Stationsplein 240-242, 2312 AR Leiden, Netherlands
City Resort Hotel Leiden
Bargelaan 180, 2333 CW Leiden, Netherlands
Please contact education@stemcell.com for more information on seasonal rates (when applicable).
Policy
Travel Visas
Citizens may require a visa in order to enter the country as visitors. Please contact us at education@stemcell.com if you require a letter of invitation for your application. Note that travel visas may take up to 30 days to process.
United Kingdom
Netherlands
Travel Visa Requirements
CanadaUnited Kingdom
Netherlands
Cancellation
A 90% refund will be issued if notification is received in writing more than 30 days in advance of the course start date. No refund will be given with less than 30 days’ notice unless we are able to find another participant to fill the spot.
STEMCELL Technologies reserves the right to cancel the course if we do not receive a minimum number of registrations 30 days in advance of the start date. Should the course be cancelled, your payment will be refunded in full. We recommend that you purchase travel insurance with your flights to cover the possibility of the course being cancelled.
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Resources and Publications
Educational Materials (12)
Related Products
Related Products
Note on Hands-On Training Courses: STEMCELL Technologies is not currently offering this standardized in-person training course, but may again in the future. Please reach out to your representative or LiveChat agent to enquire about customized training, or contact us through our phone or email channels. In the meantime, see what Live Virtual or On-Demand courses we offer, or visit this page to learn more about our Proficiency Testing Programs.
Quality Statement:
STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.
