RoboSep™-S
Fully automated cell separator
New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more
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New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more
Products for Your Protocol
To see all required products for your protocol, please consult the
Protocols and Documentation.
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RoboSep™-S Additional Service PackagesRoboSep™-S - The Fully Automated Cell Separator
What Our Product Specialist Says
Cell isolation can be time-consuming and laborious when working with multiple samples. We developed RoboSep™ to automate this process and reduce hands-on time—to help you move your research forward.
Jo GromadzkiSenior Manager, Product Maintenance
Overview
Fully automate your cell separation with the RoboSep™-S instrument. Using EasySep™ technology, RoboSep™-S performs all steps necessary to magnetically label and separate virtually any cell type by positive or negative selection. RoboSep™-S is designed to minimize sample handling, eliminate cross-contamination, and reduce hands-on time.
Supplementary products for the operation and maintenance of this instrument are available for purchase separately and include the RoboSep™ Tip Head Polishing Compoundand the RoboSep™ Tube Kit .
For more information on our instruments for automated cell separation, please see our RoboSep™ overview, and explore cell isolation kits and accessories for use with RoboSep™.
Leasing options and warranty coverage are available. Please contact us for further information.
For more information about Instrument Services, including additional service packages and software, please see our instrumentation overview.
Supplementary products for the operation and maintenance of this instrument are available for purchase separately and include the RoboSep™ Tip Head Polishing Compoundand the RoboSep™ Tube Kit .
For more information on our instruments for automated cell separation, please see our RoboSep™ overview, and explore cell isolation kits and accessories for use with RoboSep™.
Leasing options and warranty coverage are available. Please contact us for further information.
For more information about Instrument Services, including additional service packages and software, please see our instrumentation overview.
Contains
• RoboSep™-S Base Unit (Catalog #21001)
• "The Big Easy" EasySep™ Magnets (Catalog #18001)
• RoboSep™ Service Rack (Catalog #20101)
• RoboSep™ Tube Kits (Catalog #20155)
• USB Flash Drive
• RoboSep™ User Reference Manual (Catalog #29792)
• RoboSep™ Quick Start Guide (Catalog #28943)
• 1-Year Warranty (Catalog #21200)
• "The Big Easy" EasySep™ Magnets (Catalog #18001)
• RoboSep™ Service Rack (Catalog #20101)
• RoboSep™ Tube Kits (Catalog #20155)
• USB Flash Drive
• RoboSep™ User Reference Manual (Catalog #29792)
• RoboSep™ Quick Start Guide (Catalog #28943)
• 1-Year Warranty (Catalog #21200)
Application
Cell Isolation
Brand
RoboSep
Area of Interest
Chimerism, HLA, Immunology, Stem Cell Biology
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Document Type
Product Name
Catalog #
Lot #
Language
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages
Workflow Stages for
B Cell Research
Workflow Stages for
Dendritic Cell Research
Workflow Stages for
Granulocyte Research
Workflow Stages for
Hematopoietic Stem and Progenitor Cell Research
Workflow Stages for
Total Lymphocyte Research
Workflow Stages for
Monocyte Research
Workflow Stages for
Myeloid Cell Research
Workflow Stages for
Natural Killer Cell Research
Workflow Stages for
T Cell Research
Workflow Stages for
HLA Analysis
Workflow Stages for
T Cell Engineering
Workflow Stages for
Chimerism Analysis
Resources and Publications
Educational Materials (28)
Publications (63)
Selective targeting of multiple myeloma by B cell maturation antigen (BCMA)-specific central memory CD8+ cytotoxic T lymphocytes: immunotherapeutic application in vaccination and adoptive immunotherapy.
Leukemia 2019 mar
Abstract
To expand the breadth and extent of current multiple myeloma (MM)-specific immunotherapy, we have identified various antigens on CD138+ tumor cells from newly diagnosed MM patients (n = 616) and confirmed B-cell maturation antigen (BCMA) as a key myeloma-associated antigen. The aim of this study is to target the BCMA, which promotes MM cell growth and survival, by generating BCMA-specific memory CD8+ CTL that mediate effective and long-lasting immunity against MM. Here we report the identification of novel engineered peptides specific to BCMA, BCMA72-80 (YLMFLLRKI), and BCMA54-62 (YILWTCLGL), which display improved affinity/stability to HLA-A2 compared to their native peptides and induce highly functional BCMA-specific CTL with increased activation (CD38, CD69) and co-stimulatory (CD40L, OX40, GITR) molecule expression. Importantly, the heteroclitic BCMA72-80 specific CTL demonstrated poly-functional Th1-specific immune activities [IFN-gamma/IL-2/TNF-alpha production, proliferation, cytotoxicity] against MM, which were correlated with expansion of Tetramer+ and memory CD8+ CTL. Additionally, heteroclitic BCMA72-80 specific CTL treated with anti-OX40 (immune agonist) or anti-LAG-3 (checkpoint inhibitor) display increased immune function, mainly by central memory CTL. These results provide the framework for clinical application of heteroclitic BCMA72-80 peptide, alone and in combination with anti-LAG3 and/or anti-OX40 therapy, in vaccination and/or adoptive immunotherapeutic strategies to generate long-lasting anti-tumor immunity in patients with MM or other BCMA expressing tumors.
Metabolic plasticity of HIV-specific CD8+ T cells is associated with enhanced antiviral potential and natural control of HIV-1 infection.
Nature metabolism 2019 jul
Abstract
Spontaneous control of human immunodeficiency virus (HIV) is generally associated with an enhanced capacity of CD8+ T cells to eliminate infected CD4+ T cells, but the molecular characteristics of these highly functional CD8+ T cells are largely unknown. In the present study, using single-cell analysis, it was shown that HIV-specific, central memory CD8+ T cells from spontaneous HIV controllers (HICs) and antiretrovirally treated non-controllers have opposing transcriptomic profiles. Genes linked to effector functions and survival are upregulated in cells from HICs. In contrast, genes associated with activation, exhaustion and glycolysis are upregulated in cells from non-controllers. It was shown that HIV-specific CD8+ T cells from non-controllers are largely glucose dependent, whereas those from HICs have more diverse metabolic resources that enhance both their survival potential and their capacity to develop anti-HIV effector functions. The functional efficiency of the HIV-specific CD8+ T cell response in HICs is thus engraved in their memory population and related to their metabolic programme. Metabolic reprogramming in vitro through interleukin-15 treatment abrogated the glucose dependency and enhanced the antiviral potency of HIV-specific CD8+ T cells from non-controllers.
Complexin 2 regulates secretion of immunoglobulin in antibody-secreting cells.
Immunity, inflammation and disease 2019
Abstract
INTRODUCTION Complexins (CPLXs), initially identified in neuronal presynaptic terminals, are cytoplasmic proteins that interact with the soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNARE) complex to regulate the fusion of vesicles to the plasma membrane. Although much is known about CPLX function in neuronal synaptic vesicle exocytosis, their distribution and role in immune cells are still unclear. In this study, we investigated CPLX2 knockout (KO) mice to reveal the role of CPLXs in exocytosis of lymphocytes. METHODS We examined the expression of CPLXs and SNAREs in lymphocytes. To study the effect of CPLXs on the immune system in vivo, we analyzed the immune phenotype of CPLX2 KO mice. Furthermore, antibodies secretion from the peritoneal cavity, spleen, and bone marrow cells of wild-type (WT) and CPLX2 KO mice were determined. RESULTS CPLX2 was detected in B cells but not in T cells, while other CPLXs and SNAREs were expressed at a similar level in both B and T cells. To clarify the function of CPLX2 in B lymphocytes, serum concentrations of immunoglobulin G (IgG), IgA, IgM, and IgE were measured in WT and CPLX2 KO mice using enzyme-linked immunosorbent assay. The level of IgM, which mainly consists of natural antibodies, was higher in KO mice than that in WT mice, while the levels of other antibodies were similar in both types of mice. Additionally, we found that spontaneous secretion of IgM and IgG1 was enhanced from the splenic antibody-secreting cells (ASCs) of CPLX2 KO mice. CONCLUSION Our data suggest that CPLX2 inhibits spontaneous secretion of IgM and IgG1 from splenic ASCs. This study provides new insight into the mechanism of antibody secretion of ASCs.
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Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
