Human Recombinant Flt3/Flk-2 Ligand
Fms-like tyrosine kinase 3/fetal liver kinase-2
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Overview
Flt3/Flk-2 Ligand exists as membrane-bound and soluble isoforms. Both isoforms are biologically active and signal through the class III tyrosine kinase receptor (Flt3/Flk-2, CD135; Rosnet et al.). Flt3/Flk-2 Ligand is produced by a variety of cell types, including uncommitted and committed hematopoietic cells and stromal fibroblasts, whereas expression of the Flt3/Flk-2 receptor is restricted to CD34+ hematopoietic stem and progenitor cells. Flt3/Flk-2 receptor is also expressed on leukemic cells and outside the hematopoietic system in the brain, placenta, and testis (Drexler & Quentmeier; Hannum et al.).
Data Figures

(A) The biological activity of Human Recombinant Flt3/Flk-2 Ligand was tested by its ability to promote the proliferation of OCI-AML5
cells. Cell proliferation was measured after 60 hours of culture using a fluorometric assay method. The EC50 is defined as the effective
concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the above example is 1.7 - 2.5 ng/mL.
(B) 1 μg of Human Recombinant Flt3/Flk-2 Ligand was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and
visualized by Coomassie Blue staining. Human Recombinant Flt3/Flk-2 Ligand has a predicted molecular mass of 17.6 kDa.
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
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Resources and Publications
Educational Materials (3)
Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.