Cell Isolation Technologies and Products Overview

Isolate cells in as little as 8 minutes with our widely-used cell separation systems. EasySep™, RosetteSep™, and SepMate™ are column-free cell separation technologies with fast and easy protocols. RoboSep™ instruments allow for fully automated cell isolation based on the EasySep™ column-free system. StemSep™ provides versatile cell isolation in a column-based format. Compare our technologies below.

Immunomagnetic Cell Separation Technologies

EasySep™ Fast, Easy and Column-Free Cell Isolation

Cell isolation using an EasySep™ cell separation magnet

Typical Sample Source:

PBMCs*, Splenocytes, Leukopaks*, Whole Blood*

Features/Advantages:

  • Isolate highly purified cells in as little as 8 minutes without columns
  • Cells are immediately ready for downstream analysis
  • Isolate cells from virtually any sample source or size
  • Fully automate using RoboSep™ cell separation technology

RoboSep™ Fully Automated Cell Isolation

Typical Sample Source:

PBMCs*, Splenocytes, Leukopaks*, Whole Blood*

Features/Advantages:

  • Perform sequential or simultaneous cell separation from up to 16 samples
  • Minimize sample handling and eliminate cross-contamination
  • Use fast and easy pre-programmed protocols for routine cell isolation or custom protocols for special applications

StemSep™ Column-Based Cell Isolation

Typical Sample Source:

PBMCs*, Splenocytes, Bone Marrow

Features/Advantages:

  • A wide selection of antibodies are available for custom separations
  • Cell isolation reagents can be used on other immunomagnetic column-based systems including manual and automated systems
  • Magnetic particles do not interfere with subsequent FACS analysis or other downstream applications
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We use EasySep™ because of its ease of use and the high purity that we get from the preparation. I especially like the negative selection kits because it allows us to keep the cells that we collect as undisturbed as possible.

Eric Song, MD/PhD Student

We like the reliability of the RoboSep™, the minimization/elimination of specimen handling by the tech during subset separation, and the low maintenance of the instrument. These factors are important to us with such a high throughput of samples processed.

Wendy Leong, Senior Clinical Laboratory Scientist


Immunodensity Cell Separation Technologies

RosetteSep™ Cell Isolation Directly From Whole Blood

Typical Sample Source:

Features/Advantages:

  • Isolate untouched cells directly from whole blood during standard density gradient centrifugation
  • No columns, magnets or special training required
  • Combine RosetteSep™ with SepMate™ to reduce centrifugation time and minimize variability

SepMate™ Hassle-Free PBMC Isolation

Typical Sample Source:

Features/Advantages:

  • Isolate PBMCs directly from whole blood in just 15 minutes
  • Avoid the need for slow and laborious sample layering over density gradient medium
  • Simply pour off PBMCs after centrifugation
  • Minimize variability between separations
  • Combine with RosetteSep™ to isolate specific cell subsets

Density Gradient Media

Cell Source:

Peripheral whole blood, cord blood, bone marrow

For Enrichment of

Mononuclear cells (MNCs)

Density

1.077 g/mL

Cost-effective Alternative to Ficoll® Compatible with

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*Certain products are only available in select territories. Please contact your local Sales representative or the Product & Scientific Support team at techsupport@stemcell.com for further information.


I tested the SepMate™ tubes yesterday and they worked… fantastically! I ran in parallel the same sample with my standard protocol and the cell recovery was the same (in a quarter of the time, of course).

Esperanza Perucha, PhD, King’s College London

Unsure which cell separation technology to choose? We can help. Contact us by phone or email or use the LiveChat function on this page to discuss your specific needs and applications.


Scientific Resources

Wallchart
Frequencies and Percentages of Mouse Immune Cell Types
Video
How to Isolate Cells with EasySep™ Column-Free Cell Separation Technology
Wallchart
Frequencies of Cell Types in Human Peripheral Blood
Brochure
Tools For Your Immunology Research

Overview of Cell Separation

Learn everything you need to know about cell separation.

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Cell Separation Methods and Techniques

Use one of these techniques to isolate cells from your sample.

Compare Methods >


Contact STEMCELL Technologies

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