Chat with an Expert

Cell Isolation Using Density Gradient Centrifugation

Density gradient centrifugation is commonly used for the isolation of specific cell populations from whole blood. This technique takes advantage of the differences in density between the various leukocytes and the density gradient medium. As a result, the particular cell populations isolated will depend on the density of the medium that is used.

To isolate mononuclear cells (MNCs) from peripheral blood, cord blood and bone marrow, it is recommended to use media with a density of 1.077 g/mL, such as Lymphoprep™ or Ficoll®. For this particular application, diluted blood is layered on top of the density gradient medium and the samples are centrifuged. Granulocytes and erythrocytes have a higher density than mononuclear cells and during centrifugation they sediment through the density medium layer. The less dense mononuclear cells remain at the plasma-density medium interface. This process is greatly facilitated by performing the density gradient centrifugation in a SepMate™ tube. For more details on how to perform this technique watch this video on how to isolate MNCs from whole blood using density gradient centrifugation.

Density Gradient Media

Lymphoprep™

Density

1.077 g/mL

For Enrichment of

Mononuclear Cells (MNCs)

Typical Sample Source

Peripheral Whole Blood, Cord Blood, Bone Marrow

Cost-effective Alternative to Ficoll® Compatible with

RosetteSep™ DM-M Density Medium

Density

1.085 g/mL

For Enrichment of

Human Myeloid Cells

Typical Sample Source

Peripheral Whole Blood

Recommended for

Isolation using the following RosetteSep™ cocktail:

HLA Myeloid Cell Enrichment Kit (Catalog #15272HLA)

Use with SepMate™
Expand Details
Collapse Details

Other Products for Cell Isolation Using Density Gradient Centrifugation

Isolate PBMCs in Just 15 Minutes with SepMate™

Isolate PBMCs in Just 15 Minutes with SepMate™

Speed up, standardize and simplify the isolation of peripheral blood mononuclear cells (PBMCs) by density gradient centrifugation with SepMate™ . This specialized tube contains an insert that prevents the sample and density media from mixing so you can isolate PBMCs in just 15 minutes.

Isolate Cell Subsets in One Single Spin with RosetteSep™

Isolate Cell Subsets in One Single Spin with RosetteSep™

Isolate a specific subset directly from whole blood during the density gradient centrifugation process with RosetteSep™. By crosslinking unwanted cells to red blood cells (RBCs) present in the sample, the desired cells are purified during standard density gradient centrifugation. RosetteSep™ is easily combined with SepMate™ to increase sample throughput and eliminate user errors associated with improper sample layering or PBMC harvesting.

Copyright © 2018 by STEMCELL Technologies Inc. All rights reserved including graphics and images. STEMCELL Technologies & Design, STEMCELL Shield Design, Scientists Helping Scientists, AGGREWELL, CELLADHERE, CLONACELL, EASYPLATE, EASYSEP, ENDOCULT, EPICULT, ESCULT, ERYTHROCLEAR, FRESR, MAMMOCULT, MEGACULT, MESENCULT, METHOCULT, MYELOCULT, NEUROCULT, PNEUMACULT, PROSTACULT, RELESR, ROBOSEP, ROSETTESEP, SEPMATE, SMARTDISH, SPINSEP, STEMDIFF, STEMSEP, STEMSPAN, and STEMVISION are trademarks of STEMCELL Technologies Canada Inc. All other trademarks and registered trademarks are the property of their respective holders. While STEMCELL has made all reasonable efforts to ensure that the information provided by STEMCELL and its suppliers is correct, it makes no warranties or representations as to the accuracy or completeness of such information.