⦁ Optimized for differentiation from neuronal progenitor cells generated using STEMdiff™ SMADi Neural Induction Kit
⦁ Enables reproducible maturation of cortical-type astrocytic precursors derived from multiple human ES and iPS cell lines
- STEMdiff™ Astrocyte Maturation Basal Medium, 80 mL
- STEMdiff™ Astrocyte Maturation Supplement A, 20 mL
- STEMdiff™ Astrocyte Maturation Supplement B, 1 mL
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Data and Publications
Figure 1. Schematic for the Embryoid Body Protocol
Cortical-type astrocytes can be generated from astrocyte precursors after 20 days in STEMdiff™ Astrocyte Differentiation Medium. For differentiation of precursors from embryonic and induced pluripotent stem cells, see the PIS.
Figure 2. Schematic for the Monolayer Protocol
Cortical-type astrocytes can be generated from astrocyte precursors after 21 days in STEMdiff™ Astrocyte Differentiation Medium. For differentiation of precursors from embryonic and induced pluripotent stem cells, see the PIS.
Figure 3. Cortical-Type Astrocytes Are Generated After Culture in STEMdiff™ Astrocyte Differentiation and Maturation Kits
NPCs generated from hPSCs in TeSR™-E8™ using the STEMdiff™ SMADi Neural Induction Kit embryoid body (EB) protocol were differentiated and matured to cortical-type astrocytes using the STEMdiff™ Astrocyte Differentiation and Maturation Kits. Cortical-type astrocytes were formed after iPS cell-derived NPCs were cultured with the STEMdiff™ Astrocyte Differentiation Kit for 3 weeks and STEMdiff™ Astrocyte Maturation Kit for 3 weeks. (A) Nuclei are labeled with DAPI (gray). The resulting cultures contain a highly pure population of astrocytes, which are (B) more than 60% GFAP-positive (green) and (C) more than 70% S100B-positive (magenta), with (D) fewer than 15% neurons (DCX-positive cells, cyan). Scale bar = 100 μm.