Defined, feeder-free maintenance medium for human ES and iPS cells

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Defined, feeder-free maintenance medium for human ES and iPS cells
From: 326 CAD


mTeSR™1 is the most widely published feeder-free cell culture medium for human embryonic stem cells (ES cells) and induced pluripotent stem cells (iPS cells), with established protocols for applications ranging from derivation to differentiation. It has been used to successfully maintain hundreds of ES and iPS cell lines in over 40 countries, and has supported top pluripotent stem cell publications and researchers. mTeSR™1 is a highly specialized, serum-free, and complete cell culture medium. With pre-screened raw materials that ensure batch-to-batch consistency and robust feeder-free protocols for ES and iPS cell culture, mTeSR™1 provides more consistent cultures with homogeneous, undifferentiated phenotypes.
  • mTeSR™1 Complete Kit (Catalog #05850)
    • mTeSR™1 Basal Medium, 400 mL (Catalog #05851)
    • mTeSR™1 5X Supplement, 100 mL (Catalog #05852)
  • mTeSR™1 Complete Kit, 1 L (Catalog #05857)
    • mTeSR™1 Basal Medium, 800 mL (Catalog #05871)
    • mTeSR™1 5X Supplement, 100 mL, 2 Bottles (Catalog #05852)
  • mTeSR™1 Complete Kit, 10 Pack (Catalog #05870)
    • mTeSR™1 Basal Medium, 400 mL, 10 Bottles (Catalog #05851)
    • mTeSR™1 5X Supplement, 100 mL, 10 Bottles (Catalog #05852)
  • mTeSR™1 Complete Kit, 25 Pack (Catalog #05875)
    • mTeSR™1 Basal Medium, 400 mL, 25 Bottles (Catalog #05871)
    • mTeSR™1 5X Supplement, 100 mL, 25 Bottles (Catalog #05852)
Specialized Media
Cell Type:
Pluripotent Stem Cells
Cell Culture; Expansion; Maintenance
Area of Interest:
Stem Cell Biology
Defined; Serum-Free

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This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications


Morphology of hESCs and hiPSCs Cultured in mTeSR™1

Figure 1. Morphology of hESCs and hiPSCs Cultured in mTeSR™1

H1 hESCs grow as colonies with (A) defined edges and (B) high nucleus-to-cytoplasm ratio. hiPSC lines (C) iPSC(IMR90)-3 and (D) MSC-iPSC1 maintained in mTeSR™1 show similar morphological characteristics. hiPSC photographs courtesy of M. O'Connor and C. Eaves, The Vancouver Human Embryonic Stem Cell Core Facility.

Human Embryonic Stem Cells Cultured in mTeSR™1 Retain Normal Karyotype Following Long-Term Passage

Figure 2. Human Embryonic Stem Cells Cultured in mTeSR™1 Retain Normal Karyotype Following Long-Term Passage

Chromosomal analysis of H1 hESCs cultured in mTeSR™1 for 48 passages shows that normal karyotype is retained during long-term passaging. Data from Dr. T Ludwig, WiCell Research Institute.

Human Embryonic Stem Cell Cultures in mTeSR™1 Are Pluripotent

Figure 3. Human Embryonic Stem Cell Cultures in mTeSR™1 Are Pluripotent

H9 hESCs were cultured for 6 passages in mTeSR™1 then injected subcutaneously into immunocompromised mice. The resulting teratoma contained cell types from all 3 germ layers. Representative tissue types are shown.

Human Embryonic Stem Cells Cultured in mTeSR™1 Express High Levels of Pluripotent Markers and Low Levels of Differentiation Markers

Figure 4. Human Embryonic Stem Cells Cultured in mTeSR™1 Express High Levels of Pluripotent Markers and Low Levels of Differentiation Markers

Flow cytometric analysis of H9 hESCs maintained in mTeSR™1 for 17 passages.


Biotechnology progress 2016 May

Serum Replacement with Albumin-Associated Lipids Prevents Excess Aggregation and Enhances Growth of Induced Pluripotent Stem Cells in Suspension Culture.

Horiguchi I et al.


Suspension culture systems are currently under investigation for the mass production of pluripotent stem (PS) cells for tissue engineering; however, the control of cell aggregation in suspension culture remains challenging. Existing methods to control aggregation such as microwell culture are difficult to scale up. To address this issue, in this study we describe a novel method that incorporates the addition of KnockOut Serum Replacement (KSR) to the PS cell culture medium. The method regulated cellular aggregation and significantly improved cell growth (a 2- to 10-fold increase) without any influence on pluripotency. In addition, we identified albumin-associated lipids as the major working ingredient of KSR responsible for this inhibition of aggregation. This is one of the simplest methods described to date to control aggregation and requires only chemically synthesizable reagents. Thus, this method has the potential to simplify the mass production process of PS cells and thus lower their cost. This article is protected by copyright. All rights reserved.
Stem cells and development 2016 May

CXCR2 Inhibition in Human Pluripotent Stem Cells Induces Predominant Differentiation to Mesoderm and Endoderm through Repression of mTOR, β-catenin, and hTERT Activities.

Jung J et al.


On the basis of our previous report verifying that CXCR2 ligands in human placenta-conditioned medium (hPCCM) support human pluripotent stem cell (hPSC) propagation without exogenous bFGF, this study was designed to identify the effect of CXCR2 manipulation on the fate of hPSCs and the underlying mechanism, which had not been previously determined. We observed that CXCR2 inhibition in hPSCs induces predominant differentiation to mesoderm and endoderm with concomitant loss of hPSC characteristics and accompanying decreased expression of mTOR, β-catenin, and hTERT. These phenomena are recapitulated in hPSCs propagated in conventional culture conditions including bFGF as well as those in hPCCM without exogenous bFGF, suggesting that the action of CXCR2 on hPSCs might not be associated with a bFGF-related mechanism. In addition, the specific CXCR2 ligand GROα markedly increased the expression of ectodermal markers in differentiation-committed embryoid bodies derived from hPSCs. This finding suggests that CXCR2 inhibition in hPSCs prohibits the propagation of hPSCs and leads to predominant differentiation to mesoderm and endoderm owing to the blockage of ectodermal differentiation. Taken together, our results indicate that CXCR2 preferentially supports the maintenance of hPSC characteristics as well as facilitates ectodermal differentiation after the commitment to differentiation, and that the mechanism might be associated with mTOR, β-catenin, and hTERT activities.
Cytometry. Part A : the journal of the International Society for Analytical Cytology 2016 May

Label-free biomarkers of human embryonic stem cell differentiation to hepatocytes.

Tsikritsis D et al.


Four different label-free, minimally invasive, live single cell analysis techniques were applied in a quantitative comparison, to characterize embryonic stem cells and the hepatocytes into which they were differentiated. Atomic force microscopy measures the cell's mechanical properties, Raman spectroscopy measures its chemical properties, and dielectrophoresis measures the membrane's capacitance. They were able to assign cell type of individual cells with accuracies of 91% (atomic force microscopy), 95.5% (Raman spectroscopy), and 72% (dielectrophoresis). In addition, stimulated Raman scattering (SRS) microscopy was able to easily identify hepatocytes in images by the presence of lipid droplets. These techniques, used either independently or in combination, offer label-free methods to study individual living cells. Although these minimally invasive biomarkers can be applied to sense phenotypical or environmental changes to cells, these techniques have most potential in human stem cell therapies where the use of traditional biomarkers is best avoided. Destructive assays consume valuable stem cells and do not characterize the cells which go on to be used in therapies; whereas immunolabeling risks altering cell behavior. It was suggested how these four minimally invasive methods could be applied to cell culture, and how they could in future be combined into one microfluidic chip for cell sorting. 2016 International Society for Advancement of Cytometry.
Advanced healthcare materials 2016 May

Enhanced Lineage-Specific Differentiation Efficiency of Human Induced Pluripotent Stem Cells by Engineering Colony Dimensionality Using Electrospun Scaffolds.

Maldonado M et al.


Electrospun scaffolds with varied stiffness promote distinct colony morphology of human induced pluripotent stem cells, which affects their subsequent differentiation. On soft scaffolds, induced pluripotent stem cells develop 3D colonies due to the pliability of the electrospun fibrous networks, leading to greater differentiation tendency to ectodermal lineage.
Development (Cambridge, England) 2016 May

Asialoglycoprotein receptor 1 is a specific cell-surface marker for isolating hepatocytes derived from human pluripotent stem cells.

Peters D et al.


Hepatocyte-like cells (HLCs) are derived from human pluripotent stem cells (hPSCs) in vitro, but differentiation protocols commonly give rise to a heterogeneous mixture of cells. This variability confounds the evaluation of in vitro functional assays performed using HLCs. Increased differentiation efficiency and more accurate approximation of the in vivo hepatocyte gene expression profile would improve the utility of hPSCs. Towards this goal, we demonstrate the purification of a subpopulation of functional HLCs using the hepatocyte surface marker asialoglycoprotein receptor 1 (ASGR1). We analyzed the expression profile of ASGR1-positive cells by microarray, and tested their ability to perform mature hepatocyte functions (albumin and urea secretion, cytochrome activity). By these measures, ASGR1-positive HLCs are enriched for the gene expression profile and functional characteristics of primary hepatocytes compared with unsorted HLCs. We have demonstrated that ASGR1-positive sorting isolates a functional subpopulation of HLCs from among the heterogeneous cellular population produced by directed differentiation.
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