IntestiCult™-SF Organoid Growth Medium (Human)

Serum-free and conditioned medium-free cell culture medium for establishment and maintenance of human intestinal organoids
IntestiCult™-SF Organoid Growth Medium (Human)

Serum-free and conditioned medium-free cell culture medium for establishment and maintenance of human intestinal organoids

100 mL
Catalog # 100-0340
539 USD
Required Products
  1. Gentle Cell Dissociation Reagent
    Gentle Cell Dissociation Reagent

    cGMP, enzyme-free cell dissociation reagent

  2. DMEM/F-12 with 15 mM HEPES
    DMEM/F-12 with 15 mM HEPES

    Dulbecco's Modified Eagle's Medium/Nutrient Ham's Mixture F-12 (DMEM/F-12) with 15 mM HEPES buffer

  3. Y-27632 (Dihydrochloride)
    Y-27632

    RHO/ROCK pathway inhibitor; Inhibits ROCK1 and ROCK2

  4. D-PBS Without Ca++ and Mg++
    D-PBS (Without Ca++ and Mg++)

    Dulbecco’s phosphate-buffered saline without calcium and magnesium

Overview

IntestiCult™-SF Organoid Growth Medium (Human) is a complete cell culture medium for efficient establishment and long-term maintenance of organoids derived from human intestinal crypts. IntestiCult™-SF is a serum-free and conditioned medium-free formulation.

Intestinal organoids provide a convenient in vitro organotypic culture system for studying the intestinal epithelium. Isolated intestinal crypts rapidly form complex organoids when cultured in IntestiCult™-SF. The organoids incorporate a functional lumen enclosed by a polarized epithelial cell layer containing known cell types of the adult intestinal epithelium. Maturing intestinal organoids establish protruding buds, resembling intestinal crypts of the in vivo tissues. IntestiCult™-SF supports the proliferation of intestinal epithelial stem cells from patient’s biopsies and expansion of organoid cultures derived from fresh tissue or from already established frozen stocks.

Applications of intestinal organoid cultures include studying the development and function of intestinal epithelium, modeling intestinal diseases, and performing targeted molecule screens. Intestinal organoid cultures can also be used for investigation of adult stem cell properties and for regenerative therapy approaches, and can be further differentiated using IntestiCult™ Organoid Differentiation Medium (Human; Catalog #100-0214).
Advantages
• Convenient, in vitro system that recapitulates many key characteristics of the adult intestinal epithelium, including intra- and intercellular signaling, self-propagating stem cell niche, and functional transport into and out of the lumen
⦁ Serum- and conditioned medium-free formulation
⦁ Suitable for use in genome editing protocols
⦁ Enables generation of intestinal organoids in one week
⦁ Complete medium formulation that delivers consistent results
Subtype
Specialized Media
Cell Type
Intestinal Cells
Species
Human
Application
Organoid Culture
Brand
IntestiCult
Area of Interest
Disease Modeling, Drug Discovery and Toxicity Testing, Epithelial Cell Biology, Organoids, Stem Cell Biology
Formulation
Serum-Free

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
IntestiCult™-SF Organoid Growth Medium (Human)
Catalog #
100-0340
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
IntestiCult™-SF Organoid Growth Medium (Human)
Catalog #
100-0340
Lot #
All
Language
English

Educational Materials (45)

Brochure
Intestinal Organoids
Brochure
Organoids
Brochure
2019-2020 Cell Culture Training Catalog
Technical Bulletin
Culturing Cancer-Derived Organoids Using IntestiCult™ Organoid Growth Medium (Human)
Technical Bulletin
Forskolin-Induced Swelling of Human Intestinal Organoids Grown in IntestiCult™
Protocol
How to Establish Intestinal Organoid Culture from Isolated Mouse Intestinal Crypts
Protocol
How to Passage Mouse Intestinal Organoids
Protocol
CRISPR-Cas9 Genome Editing of Human Intestinal Organoids
Protocol
Performing Immunocytochemical Staining of Epithelial Organoids
Protocol
How to Isolate Mouse Intestinal Crypts
Wallchart
SnapShot: Growing Organoids from Stem Cells
Wallchart
SnapShot: The Intestinal Crypt
Wallchart
SnapShot: GI Tract Development
Video
Growth of Liver Organoids in HepatiCult™ Culture Medium: Under a Microscope
1:18
Growth of Liver Organoids in HepatiCult™ Culture Medium: Under a Microscope
Video
How to Count Intestinal Crypts for Mouse Organoid Cultures
1:09
How to Count Intestinal Crypts for Mouse Organoid Cultures
Video
Why Culture Intestinal Organoids with IntestiCult™ Organoid Growth Medium
3:19
Why Culture Intestinal Organoids with IntestiCult™ Organoid Growth Medium
Video
Collaborating to Accelerate COVID-19 Research
3:36
Collaborating to Accelerate COVID-19 Research
Video
Growth of Mouse Pancreatic Organoids in PancreaCult™ Culture Medium: Under a Microscope
1:07
Growth of Mouse Pancreatic Organoids in PancreaCult™ Culture Medium: Under a Microscope
Webinar
Adding Hepatic Organoids to Your Research
50:59
Adding Hepatic Organoids to Your Research
Webinar
Nature Research Round Table: Organoids as an Enabling Technology for Precision Cancer Medicine
17:20
Nature Research Round Table: Organoids as an Enabling Technology for Precision Cancer Medicine
Webinar
Nature Research Round Table: Organoid Modeling of Stem Cells and Disease Microenvironments
4:47
Nature Research Round Table: Organoid Modeling of Stem Cells and Disease Microenvironments
Webinar
Nature Research Round Table: Modeling Intestinal Development In Vivo and In Vitro
10:17
Nature Research Round Table: Modeling Intestinal Development In Vivo and In Vitro
Webinar
Challenges in Translating iPSC Technology
41:47
Challenges in Translating iPSC Technology
Webinar
Organoid Expert Panel
52:35
Organoid Expert Panel
Webinar
HUB & STEMCELL Organoids as Models of Infectious Disease Mini-Symposium
1:10:09
HUB & STEMCELL Organoids as Models of Infectious Disease Mini-Symposium
Webinar
Modeling Human Gastrointestinal Development and Disease Using Pluripotent Stem Cells
56:31
Modeling Human Gastrointestinal Development and Disease Using Pluripotent Stem Cells
Webinar
Nature Research Round Table: Modeling Congenital Pediatric Diarrhea in Intestinal Enteroids
15:15
Nature Research Round Table: Modeling Congenital Pediatric Diarrhea in Intestinal Enteroids
Webinar
Nature Research Round Table: Self-Organization and Symmetry Breaking in Intestinal Organoid Development
11:52
Nature Research Round Table: Self-Organization and Symmetry Breaking in Intestinal Organoid Development
Webinar
GI Tract Organoids: Using Advanced Tissue Models to Interrogate Absorption and Regulation
24:56
GI Tract Organoids: Using Advanced Tissue Models to Interrogate Absorption and Regulation
Webinar
Spotlight on Organoids: Expert Panel
Webinar
ISSCR Innovation Showcase: Applications of Organoid and Organotypic Cultures in Infectious Diseases, Nephrotoxicity, and Highly Relevant Cell-Based Assay
59:52
ISSCR Innovation Showcase: Applications of Organoid and Organotypic Cultures in Infectious Diseases, Nephrotoxicity, and Highly Relevant Cell-Based Assay
Webinar
Patient-Derived Organoids for Drug Screening and Development
50:25
Patient-Derived Organoids for Drug Screening and Development
Webinar
Nature Research Round Table: The Promise of Organoid Medicine
17:17
Nature Research Round Table: The Promise of Organoid Medicine
Webinar
Nature Research Round Table: Liver Organoids for the Study of Liver Regeneration and Disease
12:57
Nature Research Round Table: Liver Organoids for the Study of Liver Regeneration and Disease
Webinar
Nature Research Round Table: Organoids as Models for Human Disease
15:10
Nature Research Round Table: Organoids as Models for Human Disease
Webinar
Modeling Host-Microbe Interactions Using Human Intestinal Organoids
16:31
Modeling Host-Microbe Interactions Using Human Intestinal Organoids
Mini Review
Intestinal Organoid Culture
Scientific Poster
Highly Efficient Differentiation of Human Pluripotent Stem Cells into Long-term Expandable “Mini-gut” Organoids
Scientific Poster
Modelling Pancreatic Cancer Through Pancreatic Exocrine Organoids Using PancreaCult™ Serum-free Medium
Scientific Poster
Efficient Establishment and Long-term Maintenance of 3-dimensional Human Small Intestinal and Colonic Organoids Using a Novel IntestiCult™ Organoid Growth Medium (Human)
Interview
Caroline Lindemans, MD, PhD
Interview
Luigi Aloia, PhD
Interview
Jason Spence
Interview
Heather McCauley, PhD
Interview
Tamara Zietek, PhD
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Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

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