PneumaCult™ Apical-Out Airway Organoid Medium
Overview
With PneumaCult™ Apical-Out Airway Organoid Medium, you can grow polarized airway organoids with outward-facing, differentiated ciliated cells in AggreWell™400 24-well plates (Catalog #34415)—without the use of cell culture inserts. To maximize experimental reproducibility, this medium is formulated without serum or BPE. For a complete apical-out airway organoid culture workflow, expand your HBECs or HAECs in PneumaCult™-Ex Plus Medium (Catalog #05040) prior to initiating organoid culture with PneumaCult™ Apical-Out Airway Organoid Medium.
Find more about the applications of lung organoids in our learning center.
⦁ Easy downstream processing with a Matrigel®-free protocol
⦁ Generates homogeneous organoids across donors
⦁ Suitable for high-throughput drug screening applications
- PneumaCult™ Apical-Out Airway Organoid Medium, Catalog #100-0620
- PneumaCult™ Apical-Out Airway Organoid Basal Medium, 450 mL
- PneumaCult™ Apical-Out Airway Organoid 10X Supplement, 50 mL
- PneumaCult™ Apical-Out Airway Organoid 1000X Supplement, 500 µL
- PneumaCult™ Apical-Out Airway Organoid Medium with AggreWell™400 (24-well plate), Catalog #100-0746
- PneumaCult™ Apical-Out Airway Organoid Basal Medium, 450 mL
- PneumaCult™ Apical-Out Airway Organoid 10X Supplement, 50 mL
- PneumaCult™ Apical-Out Airway Organoid 1000X Supplement, 500 µL
- AggreWell™400, 24-well Plate, 1 pack
- PneumaCult™ Apical-Out Airway Organoid Medium with AggreWell™400 (5 x 24-well plates), Catalog #100-0747
- PneumaCult™ Apical-Out Airway Organoid Basal Medium, 450 mL
- PneumaCult™ Apical-Out Airway Organoid 10X Supplement, 50 mL
- PneumaCult™ Apical-Out Airway Organoid 1000X Supplement, 500 µL
- AggreWell™400, 24-well Plate, 5 pack
Scientific Resources
Product Documentation
Educational Materials (7)
Product Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Data and Publications
Data
Figure 1. Generation of Apical-Out Airway Organoids Using the PneumaCult™ Apical-Out Airway Organoid Medium
In the two-dimensional expansion phase of the human apical-out airway organoid culture protocol, HBECs or HAECs are expanded using PneumaCult™-Ex Plus Medium. The HBECs or HAECs are then plated into an AggreWell™400 24-well plate and allowed to aggregate for 1 - 6 days using PneumaCult™ Apical-Out Airway Organoid Medium. Following the aggregation, the cells are dislodged from the microwells and the aggregate suspension is transfered to a 24-well flat-bottom plate and differentiated for 9 - 14 days using PneumaCult™ Apical-Out Airway Organoid Medium to generate apical-out airway organoids that display beating cilia.
Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
