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Mouse Recombinant IL-4

Interleukin 4

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From: 190 USD

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Interleukin 4
From: 190 USD

Overview

Interleukin 4 (IL-4) is a type I cytokine produced by Th2 T cells, NK T cells, γ/δ T cells, eosinophils, mast cells, and activated basophils. IL-4 receptors are present on a number of cells, including hematopoietic, endothelial, epithelial, muscle, and brain tissues. IL-4 receptor engagement initiates signaling through the JAK/STAT pathway, and leads to the activation of PI3K and Ras/MAPK pathways (Nelms et al.). IL-4 plays a key role in immunoglobulin class switching in B cells and in regulating the differentiation of naïve T cells into T helper 2 (Th2) cells, while inhibiting Th1 differentiation. IL-4 acts together with tumor necrosis factor to stimulate expression of adhesion molecules on vascular endothelial cells and to downregulate expression of E-selectin, thus recruiting T cells and eosinophils to the site of inflammation (Nelms et al.).
Alternative Names:
B cell stimulating factor, BCDF, BCGF, BSF-1, Interleukin-4
Cell Type:
Hematopoietic Stem and Progenitor Cells; Dendritic Cells; Monocytes; Myeloid Cells; T Cells; B Cells; Lymphocytes; Neurons
Species:
Mouse
Area of Interest:
Immunology; Stem Cell Biology; Neuroscience
Molecular Weight:
13.7 kDa
Purity:
≥ 95%

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(3)

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Data

(A) The biological activity of Mouse Recombinant IL-4 was tested by its ability to promote the proliferation of HT-2 cells. Cell proliferation was measured after 67 hours of culture using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the above example is 3.8 - 5.7 ng/mL.
(B) 1 μg of Mouse Recombinant IL-4 was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Mouse Recombinant IL-4 has a predicted molecular mass of 13.7 kDa.

STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.