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Data and Publications
(A) The biological activity of Human Recombinant DKK-1 was tested by its ability to promote alkaline phosphatase production in CCL-226 cells. Alkaline phosphatase production was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which alkaline phosphatase production is at 50% of maximum. The EC50 in the above example is less than 4 μg/mL. (B) 2 μg of Human Recombinant DKK-1 was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant DKK-1 has a predicted molecular mass of 26.6 kDa.