Matrix for maintenance of human ES and iPS cells in combination with TeSR™ maintenance media
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For consistent cell populations and reproducible results in downstream applications, use CellAdhere™ Laminin-521 with TeSR™ maintenance media to provide a defined culture substrate for cell maintenance. Laminin 521 is expressed and secreted by human pluripotent stem cells (hPSCs) in the inner cell mass of the embryo and therefore creates a biologically relevant hPSC culture environment in vitro. Compared to other matrices, CellAdhere™ Laminin-521 increases single-cell attachment and survival and does not require the addition of apoptotic inhibitors during plating.
Note that single-cell passaging of human ES and iPS cells can result in selective pressure and lead to genetic aberrations. If passaging as single cells, we recommend checking the karyotype frequently.
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Resources and Publications
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PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.