Anti-Human CD45 Antibody, Clone HI30

Mouse monoclonal IgG1 antibody against human, chimpanzee CD45
From: 83 USD

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Mouse monoclonal IgG1 antibody against human, chimpanzee CD45
From: 83 USD

Overview

The HI30 antibody reacts with all isoforms of CD45, a type I transmembrane glycoprotein expressed on the surface of most hematopoietic cells except mature erythrocytes, platelets, and plasma cells; expression of CD45 is lost during differentiation of these cell types. CD45 is a member of the protein tyrosine phosphatase family and functions in a number of immunoregulatory processes, including cell activation, growth, differentiation, and oncogenic transformation. The large cytoplasmic portion of CD45 contains two tyrosine phosphatase domains (one of which is enzymatically active) that are involved in modulating the function of intracellular substrates such as the Src kinases Lck and Fyn. Several isoforms of CD45 have been identified, the expression of which differs according to cell type and functional status. Alternative splicing of three exons (4, 5, 6) encoding the extracellular RA, RB, and RC polypeptide sequences gives rise to up to 8 isoforms with molecular masses in the range of 180 - 240 kDa. The leukocyte common antigen (LCA), the region recognized by the HI30 antibody, is an extracellular region located proximal to the membrane and common to all isoforms of CD45.

This antibody clone has been verified for purity assessments of cells isolated with EasySep™ kits, including EasySep™ Human T Cell Enrichment Kit (Catalog #19051), EasySep™ Human CD4+ T Cell Enrichment Kit (Catalog #19052) and EasySep™ Human CD45 Depletion Kit (Catalog #18259; partial blocking may be observed).
Subtype:
Primary Antibodies
Target Antigen:
CD45
Alternative Names:
B220, LCA, T200
Reactive Species:
Human; Chimpanzee
Conjugation:
Alexa Fluor 488; APC; Biotin; FITC; PE; PerCP-Cyanine5.5; Unconjugated; Pacific Blue
Host Species:
Mouse
Cell Type:
Lymphocytes; Myeloid Cells; Granulocytes and Subsets; Hematopoietic Stem and Progenitor Cells
Application:
CyTOF; Cell Isolation; Functional Assay; Flow Cytometry; Immunocytochemistry; Immunofluorescence; Immunohistochemistry; Immunoprecipitation; Western Blotting
Area of Interest:
Immunology
Clone:
HI30
Gene ID:
5788
Isotype:
IgG1, kappa

Scientific Resources

Product Documentation

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Educational Materials

(6)

Data and Publications

Data

Data for Alexa Fluor® 488-Conjugated

Figure 1. Data for Alexa Fluor® 488-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD45 Antibody, Clone HI30, Alexa Fluor® 488 (filled histogram) or a mouse IgG1, kappa Alexa Fluor® 488 isotype control antibody (solid line histogram).

Data for APC-Conjugated

Figure 2. Data for APC-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD45 Antibody, Clone HI30, APC (filled histogram) or a mouse IgG1, kappa APC isotype control antibody (solid line histogram).

Data for Biotin-Conjugated

Figure 3. Data for Biotin-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD45 Antibody, Clone HI30, Biotin, followed by streptavidin (SAV) APC (filled histogram), or a mouse IgG1, kappa biotin isotype control antibody, followed by SAV APC (solid line histogram).

Data for FITC-Conjugated

Figure 4. Data for FITC-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD45 Antibody, Clone HI30, FITC (filled histogram) or a mouse IgG1, kappa FITC isotype control antibody (solid line histogram).

Data for PE-Conjugated

Figure 5. Data for PE-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD45 Antibody, Clone HI30, PE (filled histogram) or a mouse IgG1, kappa PE isotype control antibody (solid line histogram).

Data for Unconjugated

Figure 6. Data for Unconjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD45 Antibody, Clone HI30, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (Catalog #60138FI) (filled histogram), or a mouse IgG1, kappa isotype control antibody, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (solid line histogram).

Data for PerCP-Cy55-Conjugated

Figure 7. Data for PerCP-Cy55-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD45 Antibody, Clone HI30, PerCP-Cy5.5 (filled histogram) or a mouse IgG1, kappa isotype control antibody, PerCP-Cy5.5 (solid line histogram).

Figure 8. Data for PB-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD45 Antibody, Clone HI30, Pacific Blue™ (filled histogram) or a mouse IgG1, kappa Pacific Blue™ isotype control antibody (solid line histogram).

STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.