Anti-Human CD3 Antibody, Clone UCHT1

Mouse monoclonal IgG1 antibody against human, chimpanzee CD3
From: 62 USD

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Mouse monoclonal IgG1 antibody against human, chimpanzee CD3
From: 62 USD

Overview

The UCHT1 antibody reacts with the ~20 kDa CD3ε subunit of the human T cell receptor (TCR)/CD3 complex, which is expressed on the surface of ~95% of mature T cells and NKT cells, and variably on thymocytes. A majority of T cell neoplasms also express CD3. The CD3 complex, which is assembled from combinations of CD3γ, δ, ε, η, and ζ subunits, associates non-covalently with the TCR and is involved in transducing antigen recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR. Activation of T cells by the TCR involves the cytoplasmic tails of the CD3 subunits, which are structurally related type 1 transmembrane proteins and members of the immunoglobulin superfamily. Mutations in the CD3 subunits have been associated with various immunodeficiency disorders including severe combined immunodeficiency (SCID).

This antibody clone has been verified for purity assessments of cells isolated with EasySep™ kits, including EasySep™ Direct Human T Cell Isolation Kit (Catalog #19661), EasySep™ Human CD3 Positive Selection Kit II (Catalog #17851; partial blocking may be observed), EasySep™ HLA Whole Blood T Cell Enrichment Kit (Catalog #19951HLA) and EasySep™ HLA Whole Blood CD2 Positive Selection Kit (Catalog #18687HLA).
Subtype:
Primary Antibodies
Target Antigen:
CD3
Alternative Names:
CD3e, CD3epsilon, T3
Reactive Species:
Human; Chimpanzee
Conjugation:
Alexa Fluor 488; APC; Biotin; FITC; PE; PerCP; PerCP-Cyanine5.5; Unconjugated
Host Species:
Mouse
Cell Type:
T Cells
Application:
Cell Isolation; Functional Assay; Flow Cytometry; Immunocytochemistry; Immunofluorescence; Immunohistochemistry; Immunoprecipitation; CyTOF; Western Blotting
Area of Interest:
Immunology
Clone:
UCHT1
Gene ID:
915
Isotype:
IgG1, kappa

Scientific Resources

Product Documentation

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Educational Materials

(7)

Data and Publications

Data

Data for Alexa Fluor® 488-Conjugated

Figure 1. Data for Alexa Fluor® 488-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD3 Antibody, Clone UCHT1, Alexa Fluor® 488 (filled histogram) or a mouse IgG1, kappa Alexa Fluor® 488 isotype control antibody (solid line histogram).

Data for APC-Conjugated

Figure 2. Data for APC-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD3 Antibody, Clone UCHT1, APC (filled histogram) or a mouse IgG1, kappa APC isotype control antibody (black line histogram).
(B) Flow cytometry analysis of human buffy coat nucleated cells processed with the EasySep™ HLA Whole Blood CD3 Positive Selection Kit and labeled with Anti-Human CD3 Antibody, Clone UCHT1, APC. Histograms show labeling of buffy coat nucleated cells (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa APC isotype control antibody is shown (open histogram).
(C) Flow cytometry analysis of human whole blood nucleated cells processed with the EasySep™ HLA Whole Blood T Cell Enrichment Kit (Catalog #19951HLA). Histograms show labeling of HetaSep™-treated whole blood cells (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa APC isotype control antibody is shown (open histogram).

Data for Biotin-Conjugated

Figure 3. Data for Biotin-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD3 Antibody, Clone UCHT1, Biotin followed by streptavidin (SAV) APC (filled histogram) or a mouse IgG1, kappa biotin isotype control antibody followed by SAV APC (black line histogram).
(B) Flow cytometry analysis of human buffy coat nucleated cells processed with the EasySep™ HLA Whole Blood CD2 Positive Selection Kit and labeled with Anti-Human CD3 Antibody, Clone UCHT1, Biotin followed by streptavidin (SAV) APC. Histograms show labeling of buffy coat nucleated cells (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa biotin isotype control antibodyfollowed by SAV APC is shown (open histogram).
(C) Flow cytometry analysis of human buffy coat nucleated cells processed with the EasySep™ HLA Whole Blood CD3 Positive Selection Kit and labeled with Anti-Human CD3 Antibody, Clone UCHT1, Biotin followed by streptavidin (SAV) APC. Histograms show labeling of buffy coat nucleated cells (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa biotin isotype control antibodyfollowed by SAV APC is shown (open histogram).

Data for FITC-Conjugated

Figure 4. Data for FITC-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD3 Antibody, Clone UCHT1, FITC (filled histogram) or a mouse IgG1, kappa FITC isotype control antibody (black line histogram).
(B) Flow cytometry analysis of human whole blood nucleated cells processed with the EasySep™ HLA Whole Blood T Cell Enrichment Kit and labeled with Anti-Human CD3 Antibody, Clone UCHT1, FITC. Histograms show labeling of HetaSep™-treated whole blood cells (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa FITC isotype control antibody is shown (open histogram).
(C) Flow cytometry analysis of human buffy coat nucleated cells processed with the EasySep™ HLA Whole Blood CD3 Positive Selection Kit and labeled with Anti-Human CD3 Antibody, Clone UCHT1, FITC. Histograms show labeling of buffy coat nucleated cells (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa FITC isotype control antibody is shown (open histogram).

Data for PE-Conjugated

Figure 5. Data for PE-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD3 Antibody, Clone UCHT1, PE (filled histogram) or a mouse IgG1, kappa PE isotype control antibody (solid line histogram).

Data for Unconjugated

Figure 6. Data for Unconjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD3 Antibody, Clone UCHT1, followed by goat anti-mouse IgG, FITC (filled histogram). Labeling with a mouse IgG1, kappa isotype control antibody followed by goat anti-mouse IgG, FITC is shown (solid line histogram).
(B) Flow cytometry analysis of PBMCs prior to being processed with EasySep™ Human CD3 Positive Selection Kit (Catalog #18051), which uses Anti-Human CD3 Antibody, Clone UCHT1, to select CD3+ cells. The cells were labeled with Anti-Human CD4 Antibody, Clone OKT4, Alexa Fluor® 488 (Catalog #60016AD) and Anti-Human CD8 Antibody, Clone RPA-T8, PE (Catalog #60022PE).
(C) Flow cytometry analysis of PBMCs processed with EasySep™ Human CD3 Positive Selection Kit and labeled with Anti-Human CD4 Antibody, Clone OKT4, Alexa Fluor® 488 (Catalog #60016AD) and Anti-Human CD8 Antibody, Clone RPA-T8 (Catalog #60022PE).

Data for PerCP-Cy55-Conjugated

Figure 7. Data for PerCP-Cy55-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD3 Antibody, Clone UCHT1, PerCP-Cy5.5 (filled histogram) or a mouse IgG1, kappa isotype control antibody, PerCP-Cy5.5 (solid line histogram).
(B) Flow cytometry analysis of human PBMCs processed with the EasySep™ Human CD3 Positive Selection Kit (Catalog #18051) and labeled with Anti-Human CD3 Antibody, Clone UCHT1, PerCP-Cy5.5. Histograms show labeling of PBMCs (Start) and isolated cells (Isolated). Labeling of start cells with a mouse IgG1, kappa isotype control antibody, PerCP-Cy5.5 is shown in the bottom panel (solid line histogram).

Data for PerCP-Cy55-Conjugated

Figure 8. Data for PerCP-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Anti-Human CD3 Antibody, Clone UCHT1, PerCP (filled histogram) or a mouse IgG1, kappa isotype control antibody, PerCP (solid line histogram).

STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.