DNase I

For digestion of DNA

DNase I

For digestion of DNA

From: 149 USD
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For digestion of DNA
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Product Advantages


  • Effectively eliminate DNA contaminants from dissociation medium


Overview

Use Deoxyribonuclease I (DNase I), Animal Component-Free (ACF) for routine tissue dissociation, to minimize cell clumping, and eliminate DNA contaminants from dissociation medium. This endonuclease consists of a single glycosylated polypeptide chain with two disulfide bonds. It preferentially cleaves phosphodiester linkages adjacent to pyrimidine nucleotides in both single- and double-stranded DNA, yielding polynucleotides with 5’-phosphate and 3’-hydroxyl groups (Bernardi et al.). DNase I has been used for DNA digestion in human cells and tissues such as microglia (Klegeris & McGeer), cartilage (Dunham & Koch), colon (Fukushima & Fiocchi), epithelium (Fukushima & Fiocchi), liver (Vatakis et al.), lung (Fujino et al.), neural cells (Fuja et al.), and stem cells (Kusuma et al.).
Subtype
Enzymatic
Alternative Names
DNA endonuclease; DNA nuclease; Deoxyribonucleic phosphatase; Pancreatic DNase; Thymonuclease
Cell Type
B Cells, Endothelial Colony Forming Cells (ECFCs), Neurons, Osteoblasts, T Cells
Species
Human, Mouse, Non-Human Primate, Other, Rat
Application
Cell Culture
Area of Interest
Cancer, Endothelial Cell Biology, Epithelial Cell Biology, Immunology, Neuroscience, Stem Cell Biology

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Product Name
DNase I
Catalog #
07469, 100-0683, 07470
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
DNase I
Catalog #
07469, 100-0683, 07470
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Resources and Publications

Educational Materials (1)