DNase I, ACF

Animal component-free DNase I for digestion of DNA
Catalog #
07473_C
Animal component-free DNase I for digestion of DNA
From: 162 USD

Overview

Deoxyribonuclease I (DNase I), Animal Component-Free (ACF) is obtained from cultures free of animal-derived materials and purified by chromatography. DNase I is an endonuclease consisting of a single glycosylated polypeptide chain with two disulfide bonds. DNase is often included in tissue dissociation protocols to digest DNA that has leaked into the dissociation medium as a result of cell damage. DNase I, ACF preferentially cleaves phosphodiester linkages adjacent to pyrimidine nucleotides in both single- and double-stranded DNA, yielding polynucleotides with 5’-phosphate and 3’-hydroxyl groups (Bernardi et al.). DNase I has been used for the digestion of human tissues such as microglia (Klegeris & McGeer), cartilage (Dunham & Koch), colon (Fukushima & Fiocchi), epithelium l (Fukushima & Fiocchi), liver (Vatakis et al.), lung (Fujino et al.), neural (Fuja et al.), and stem cells (Kusuma et al.).
Subtype
Enzymatic
Alternative Names
DNA endonuclease; DNA nuclease; Deoxyribonucleic phosphatase; Pancreatic DNase; Thymonuclease
Cell Type
Airway Cells, Chondrocytes, Mesenchymal Stem and Progenitor Cells, Neurons
Species
Human, Mouse, Rat, Non-Human Primate, Other
Area of Interest
Cancer, Epithelial Cell Biology, Immunology, Neuroscience, Stem Cell Biology
Molecular Weight
29.1 kDa

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
DNase I, ACF
Catalog #
07473, 07474, 100-0273
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
DNase I, ACF
Catalog #
07473, 07474, 100-0273
Lot #
All
Language
English

Educational Materials (1)

Wallchart
Human Immune Cytokines

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

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