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DNase I, ACF

Animal component-free DNase I for digestion of DNA

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From: 134 USD

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Animal component-free DNase I for digestion of DNA
From: 134 USD

Overview

Deoxyribonuclease I (DNase I), Animal Component-Free (ACF) is obtained from cultures free of animal-derived materials and purified by chromatography. DNase I is an endonuclease consisting of a single glycosylated polypeptide chain with two disulfide bonds. DNase is often included in tissue dissociation protocols to digest DNA that has leaked into the dissociation medium as a result of cell damage. DNase I, ACF preferentially cleaves phosphodiester linkages adjacent to pyrimidine nucleotides in both single- and double-stranded DNA, yielding polynucleotides with 5’-phosphate and 3’-hydroxyl groups (Bernardi et al.). DNase I has been used for the digestion of human tissues such as microglia (Klegeris & McGeer), cartilage (Dunham & Koch), colon (Fukushima & Fiocchi), epithelium l (Fukushima & Fiocchi), liver (Vatakis et al.), lung (Fujino et al.), neural (Fuja et al.), and stem cells (Kusuma et al.).
Subtype:
Enzymatic
Alternative Names:
DNA endonuclease; DNA nuclease; Deoxyribonucleic phosphatase; Pancreatic DNase; Thymonuclease
Cell Type:
Airway Cells; Chondrocytes; Mesenchymal Stem and Progenitor Cells; Neurons
Species:
Human; Mouse; Rat; Non-Human Primate; Other
Area of Interest:
Cancer Research; Epithelial Cell Biology; Immunology; Neuroscience; Stem Cell Biology
Molecular Weight:
29.1 kDa

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STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.