DNase I, ACF

Animal component-free DNase I for digestion of DNA

DNase I, ACF

Animal component-free DNase I for digestion of DNA

From: 189 USD
Catalog #
07473_C
Animal component-free DNase I for digestion of DNA
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Overview

Deoxyribonuclease I (DNase I), Animal Component-Free (ACF) is obtained from cultures free of animal-derived materials and purified by chromatography. DNase I is an endonuclease consisting of a single glycosylated polypeptide chain with two disulfide bonds. DNase is often included in tissue dissociation protocols to digest DNA that has leaked into the dissociation medium as a result of cell damage. DNase I, ACF preferentially cleaves phosphodiester linkages adjacent to pyrimidine nucleotides in both single- and double-stranded DNA, yielding polynucleotides with 5’-phosphate and 3’-hydroxyl groups (Bernardi et al.). DNase I has been used for the digestion of human tissues such as microglia (Klegeris & McGeer), cartilage (Dunham & Koch), colon (Fukushima & Fiocchi), epithelium l (Fukushima & Fiocchi), liver (Vatakis et al.), lung (Fujino et al.), neural (Fuja et al.), and stem cells (Kusuma et al.).
Subtype
Enzymatic
Cell Type
Airway Cells, Chondrocytes, Mesenchymal Stem and Progenitor Cells, Neurons
Species
Human, Mouse, Non-Human Primate, Other, Rat
Area of Interest
Cancer, Epithelial Cell Biology, Immunology, Neuroscience, Stem Cell Biology

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Product Name
DNase I, ACF
Catalog #
07473, 100-0273, 07474
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
DNase I, ACF
Catalog #
07473, 100-0273, 07474
Lot #
All
Language
English

Applications

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Resources and Publications

Educational Materials (1)