Human/Mouse Recombinant Activin A, ACF

Activin A, animal component-free
Catalog #
78132_C
Activin A, animal component-free
From: 204 USD

Overview

Activin A is a member of the transforming growth factor beta (TGF-β) family of proteins produced by many cell types throughout development (Gurdon et al.). It is a disulfide-linked homodimer (two beta-A chains) that binds to heteromeric complexes of a type I (Act RI-A and Act RI-B) and a type II (Act RII-A and Act RII-B) serine-threonine kinase receptor (Attisano et al.). Activins primarily signal through SMAD2/3 proteins to regulate a variety of functions, including cell proliferation, differentiation, wound healing, apoptosis, and metabolism (McDowell et al.). Activin A maintains the undifferentiated state of human embryonic stem cells (James et al.; Xiao et al.) and also facilitates differentiation of human embryonic stem cells into definitive endoderm (D’Amour et al.). This product is animal component-free.
Subtype
Cytokines
Alternative Names
Activin beta-A chain, EDF, Erythroid differentiation protein, FRP, FSH-releasing protein, INHBA, Inhibin betaA chain, Inhibin beta-1
Cell Type
Endoderm, PSC-Derived, Mesoderm, PSC-Derived, Other, Pluripotent Stem Cells
Species
Human, Mouse
Area of Interest
Epithelial Cell Biology, Stem Cell Biology
Molecular Weight
13.1 kDa monomer; 26.2 kDa dimer
Purity
≥ 95%

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
Human/Mouse Recombinant Activin A, ACF
Catalog #
78132, 78132.1, 78132.2
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Human/Mouse Recombinant Activin A, ACF
Catalog #
78132, 78132.1, 78132.2
Lot #
All
Language
English

Educational Materials (7)

Brochure
Products for Human Pluripotent Stem Cells
Brochure
Cytokines, Chemokines and Growth Factors
Brochure
Small Molecules for Stem Cell Research
Technical Bulletin
Weekend-Free Culture of Human Pluripotent Stem Cells in mTeSR™1 or TeSR™-E8™
Wallchart
Stem Cell States: Naive to Primed Pluripotency
Wallchart
Directed Differentiation of Pluripotent Stem Cells
Webinar
Methods and Culture Reagents to Maintain a Spectrum of hPSC States from Naive-Like to Primed
23:35
Methods and Culture Reagents to Maintain a Spectrum of hPSC States from Naive-Like to Primed

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

(A) The biological activity of Human/Mouse Recombinant Activin A, ACF was tested by its ability to inhibit proliferation of MPC-11 cells. Inhibition of cell proliferation was measured after 66 hours of culture using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation inhibition is at 50% of maximum. The EC50 in the example above is 0.436 ng/mL. (B) 1 μg of Human/Mouse Recombinant Activin A, ACF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining.

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