Human Recombinant BMP-2, ACF

Bone morphogenetic protein 2, animal component-free

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Bone morphogenetic protein 2, animal component-free
From: 210 USD

Overview

Bone morphogenetic protein 2 (BMP-2) is a member of the transforming growth factor beta (TGF-β) superfamily. BMP-2 is a disulfide-linked homodimer, acts as a ligand for complexes of type I and II BMP receptors, and primarily activates SMAD1/5/8 signaling (Nohe et al.). BMP-2 is a potent differentiation factor and directs human pluripotent stem cells (hPSCs) towards various cell types including extra-embryonic endoderm, mesenchymal cells, and chondrocytes (Pera et al.). Although BMP-2 expression is low in healthy cartilage, its expression is upregulated at the site of cartilage damage (Blaney Davidson et al.). BMP-2 induces bone and cartilage formation in vitro and is able to induce chondrogenesis in human mesenchymal stem cells (Schmitt et al.). This product is animal component-free.
Alternative Names:
BDA2, BMP-2A, BMP2, Bone morphogenetic protein 2A
Cell Type:
Endoderm, PSC-Derived; Hematopoietic Stem and Progenitor Cells; Mesoderm, PSC-Derived; Pluripotent Stem Cells
Area of Interest:
Epithelial Cell Biology; Neuroscience; Stem Cell Biology
Molecular Weight:
13 kDa monomer; 26.1 kDa dimer
Purity:
≥ 95%

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This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

(A) The biological activity of Human Recombinant BMP-2, ACF was tested by its ability to induce alkaline phosphatase activity in ATDC-5 cells. Cell proliferation was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which alkaline phosphatase production is at 50% of maximum. The EC50 in the example above is 74 ng/mL. (B) 1 μg of Human Recombinant BMP-2, ACF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining.

STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.