Human Recombinant RANKL

Receptor activator of nuclear factor kappa-B ligand

Human Recombinant RANKL

Receptor activator of nuclear factor kappa-B ligand

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Receptor activator of nuclear factor kappa-B ligand
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Overview

Receptor activator of nuclear factor kappa-B ligand (RANKL) is a member of the tumor necrosis factor (TNF) superfamily (Anderson et al.). Cytokines in the TNF superfamily are involved in a variety of long-term cellular activities such as differentiation, proliferation, and cell death (MacEwan). RANKL is a type II homotrimeric transmembrane protein expressed in both a membrane-bound and secreted form (Ikeda et al.). RANKL binds to the receptor activator of nuclear factor kappa-B (RANK). Upon binding to its receptor, RANKL activates the AKT signaling pathway (Moon et al.). Osteoprotegerin (OPG) may also bind RANKL, and this binding competes with RANKL-RANK binding (Lacey et al.). RANKL is involved in osteoclastogenesis (Lacey et al.; Yasuda et al.) and T cell activation (Wong et al.).
Subtype
Cytokines
Cell Type
Airway Cells, Cancer Cells and Cell Lines, Dendritic Cells, Intestinal Cells, Mammary Cells, Osteoblasts, Other, Pancreatic Cells, Prostate Cells, T Cells
Species
Human
Area of Interest
Cancer, Stem Cell Biology
Purity
≥ 95%

Data Figures

(A) The biological activity of Human Recombinant RANKL was tested by its ability to induce production of embryonic alkaline phosphatase in RAW-Blue™ macrophage reporter cells. Alkaline phosphatase production was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which alkaline phosphatase activity is at 50% of maximum. The EC50 in the above example is 38.8 ng/mL. (B) Human Recombinant RANKL was resolved with SDS-PAGE under reducing (+) conditions and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant RANKL has a predicted molecular mass of 19.7 kDa.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
78214.1, 78214, 78214.3
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
78214.1, 78214, 78214.3
Lot #
All
Language
English