Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11

Mouse monoclonal IgG2b, kappa isotype control antibody
From: 36 USD

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Mouse monoclonal IgG2b, kappa isotype control antibody
From: 36 USD

Overview

The MPC-11 antibody (IgG2b, kappa) is suitable for use as an isotype-matched control antibody in several applications to estimate the degree of non-specific binding by an antigen-specific antibody. Ideally, the isotype control should have the same subclass of heavy chain (IgA, IgD, IgE, IgG, or IgM) and light chain (kappa or lambda) as the specific antibody being used. If a conjugated antibody is used, an isotype control conjugated to the same molecule (e.g. fluorochrome) should be chosen. The use of an appropriate isotype control helps confirm the specificity of the antigen-specific antibody, and indicates non-specific binding that may result from binding to Fc receptors or other cell components.

This antibody clone has been verified for use as an isotype control antibody for assessing non-specific binding to cells in applications such as flow cytometry and immunofluorescence microscopy applications (surface and intracellular staining).
Subtype:
Isotype Controls
Target Antigen:
IgG2b Isotype Control
Alternative Names:
Not applicable
Conjugation:
Alexa Fluor 488; APC; Biotin; FITC; PE; PerCP-Cyanine5.5; Unconjugated
Host Species:
Mouse
Cell Type:
Other
Application:
ELISA; Flow Cytometry; Functional Assay; Immunocytochemistry; Immunofluorescence; Immunohistochemistry; Immunoprecipitation; Western Blotting
Area of Interest:
Immunology
Clone:
MPC-11
Gene ID:
Not applicable
Isotype:
IgG2b, kappa

Scientific Resources

Product Documentation

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Educational Materials

(3)

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Flow Cytometry Analysis of Human PBMCs

Figure 1. Flow Cytometry Analysis of Human PBMCs

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC11, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (Catalog #60138FI) (solid line histogram). Filled histogram shows labeling with a mouse IgG2b, kappa positive control antibody (Anti-Human CD20 Antibody, Clone 2H7; Catalog #60008), followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC. (B) Human induced pluripotent stem (iPS) cells were cultured in TeSR™-E8™ (Catalog #05940) on glass coverslips coated with Vitronectin XF™ (Catalog #07180), then fixed and labeled with Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11, followed by goat anti-mouse IgG, FITC (Inset), or with a positive control antibody of the same isotype, Anti-Human OCT4 (OCT3) Antibody, Clone 3A2A20 (Catalog #60093), followed by goat anti-mouse IgG, FITC (green).

Data for Alexa Fluor® 488-Conjugated

Figure 2. Data for Alexa Fluor® 488-Conjugated

(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11, Alexa Fluor® 488 (solid line histogram). Filled histogram shows labeling with a mouse IgG2b, kappa positive control antibody (Anti-Human CD20 Antibody, Clone 2H7, Alexa Fluor® 488, Catalog #60008AD). (B) Human embryonic stem (ES) cells were cultured with TeSR™-E8™ (Catalog #05940) on glass coverslips coated with Vitronectin XF™ (Catalog #07180), then fixed and stained with Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11, Alexa Fluor® 488 (inset) or with a positive control antibody of the same isotype, Anti-Human OCT4 (OCT3) Antibody, Clone 3A2A20, Alexa Fluor® 488 (Catalog #60093AD, green).

Data for APC-Conjugated

Figure 3. Data for APC-Conjugated

Flow cytometry analysis of human buffy coat nucleated cells labeled with Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11, APC (solid line histogram). Filled histogram shows labeling with a mouse IgG2b, kappa positive control antibody (Anti-Human CD20 Antibody, Clone 2H7, APC, Catalog #60008AZ).

Data for Biotin-Conjugated

Figure 4. Data for Biotin-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11, Biotin followed by streptavidin (SAV) APC (solid line histogram). Filled histogram shows labeling with a mouse IgG2b, kappa positive control antibody (Anti-Human CD20 Antibody, Clone 2H7, Biotin, Catalog #60008BT) followed by SAV APC.

Data for FITC-Conjugated

Figure 5. Data for FITC-Conjugated

Flow cytometry analysis of human buffy coat nucleated cells labeled with Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11, FITC (solid line histogram). Filled histogram shows labeling with a mouse IgG2b, kappa positive control antibody (Anti-Human CD20 Antibody, Clone 2H7, FITC, Catalog #60008FI).

Data for PE-Conjugated

Figure 6. Data for PE-Conjugated

(A) Flow cytometry analysis of human buffy coat nucleated cells labeled with Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11, PE (solid line histogram). Filled histogram shows labeling with a mouse IgG2b, kappa positive control antibody (Anti-Human CD20 Antibody, Clone 2H7, PE, Catalog #60008PE). (B) Human embryonic stem (ES) cells were cultured with TeSR™-E8™ (Catalog #05940) on glass coverslips coated with Vitronectin XF™ (Catalog #07180), then fixed and stained with Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11, PE (inset) or with a positive control antibody of the same isotype, Anti-Human OCT4 (OCT3) Antibody, Clone 3A2A20, PE (Catalog #60093PE).

Data for PerCP-Cy55-Conjugated

Figure 7. Data for PerCP-Cy55-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11, PerCP-Cy5.5 (solid line histogram). Filled histogram shows labeling with a mouse IgG2b, kappa positive control antibody (Anti-Human CD20 Antibody, Clone 2H7, PerCP-Cy5.5, Catalog #60008PS).

STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.